PMID: 7525285Nov 1, 1994Paper

Epidermal-growth-factor-dependent activation of the src-family kinases

European Journal of Biochemistry
N Osherov, A Levitzki

Abstract

The precise role of src-type kinases as signal transducers has been under intensive investigation but only in a few instances has their role been revealed in any detail. Thus, src, fyn and yes are activated upon stimulation by platelet-derived growth factor or colony-stimulating factor in cells expressing high levels of these receptors. Activation of src-family kinases by other receptor tyrosine kinases such as the epidermal-growth-factor (EGF) receptor has not been directly demonstrated. In this report, we demonstrate EGF-dependent activation of src-family tyrosine kinases in NIH3T3 cells overexpressing the human EGF receptor. Activation is rapid (< 1 min) and persistent (up to 16 h). Furthermore, we show a correlation between the level of EGF receptor expressed and the degree of src-family kinase activation. We show that src-family kinase activity is also activated by addition of EGF to PC12 cells, which endogenously express relatively high levels of EGF receptor. Most strikingly, we show that A431 cells, which endogenously express very high levels of EGF receptor, show 10-fold elevated src-family kinase activity as compared to DHER14 cells, and that this activity is constitutive. This activity is completely blocked by AG1478...Continue Reading

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Citations

May 15, 1998·Journal of Cellular Biochemistry·B Y KleinH Ben-Bassat
May 1, 1996·Journal of Cellular Physiology·M I Garcia-LloretL J Guilbert
Jun 1, 1997·Medical Oncology·A Levitzki
Jan 11, 1996·Journal of Biochemical and Biophysical Methods·P A Weernink, G Rijksen
Apr 1, 1995·Current Opinion in Cell Biology·T Erpel, S A Courtneidge
Dec 1, 1995·Current Opinion in Biotechnology·D W Fry, A J Bridges
May 20, 2004·Experimental Cell Research·Pamela E Knapp, Mary H Adams
Jun 11, 2002·Biochemical and Biophysical Research Communications·Barbara J BlanchardVernon M Ingram
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
Aug 24, 1999·Pharmacology & Therapeutics·A Levitzki
Jul 18, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Alexander Levitzki
Apr 1, 1996·Current Opinion in Cell Biology·A Levitzki
Dec 5, 2002·Breast Cancer Research and Treatment·Yasuhiro ItoNariaki Matsuura
Mar 2, 2010·Nature Chemical Biology·Jiannong LiEric B Haura
Jun 17, 2010·Nature Reviews. Clinical Oncology·Deric L WheelerPaul M Harari
Jul 18, 2002·Nature Reviews. Drug Discovery·Philip Cohen
Apr 9, 2008·Oncogene·J Lin, R Arlinghaus
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mayumi F MillerEdward E Morrisey
May 5, 2005·DNA and Cell Biology·Mark Richter, Hongtao Zhang
Mar 4, 2000·The EMBO Journal·X M ZhengD Shalloway
Jun 1, 2005·Molecular and Cellular Biology·Rotem KarniAlexander Levitzki
Jun 8, 2006·Annual Review of Biochemistry·Alexander Levitzki, Eyal Mishani
Aug 3, 2007·The Journal of Clinical Investigation·Tony Hunter

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