Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro

Head & Face Medicine
Casiano Del Angel-MosquedaMyriam Angélica De la Garza-Ramos

Abstract

Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. These data suggests that DPSCs in combination with EGF could be an effective ...Continue Reading

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Citations

Jun 26, 2018·Cytotechnology·Ibrahim Mortada, Rola Mortada
Oct 20, 2017·Journal of Applied Biomaterials & Functional Materials·Casiano Del Angel-MosquedaClaudio Cabral-Romero
Jan 22, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anowar H KhanAdam T Melvin
Mar 29, 2016·Acta Biomaterialia Odontologica Scandinavica·Martha Alicia Laredo-NaranjoRoberto Carrillo-Fuentevilla
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Feb 6, 2021·Experimental Dermatology·Georgia SotiropoulouGeorgios Pampalakis
Nov 23, 2016·Journal of Endodontics·Qian ZengMaobin Yang
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Aug 28, 2021·International Journal of Molecular Sciences·Stefano PulciniThelma A Pertinhez

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Methods Mentioned

BETA
fluorescence microscopy
light microscopy
PCR
flow cytometry

Software Mentioned

SPSS

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