PMID: 9434127Jan 20, 1998Paper

Epidermal growth factor-induced activation and translocation of c-Src to the cytoskeleton depends on the actin binding domain of the EGF-receptor

Biochimica Et Biophysica Acta
Marcel A G van der HeydenG Rijksen

Abstract

In the epidermal growth factor (EGF)-receptor signal transduction cascade, the non-receptor tyrosine kinase c-Src has been demonstrated to become activated upon EGF stimulation. In this paper we show that c-Src associates with the cytoskeleton and co-isolates with actin filaments upon EGF treatment of NIH-3T3 cells transfected with the EGF receptor. Immunofluorescence studies using CLSM show colocalization of F-actin and endogenous c-Src predominantly around endosomes and not on stress fibers and cell-cell contacts. Stimulation of EGF receptor-transfected NIH-3T3 cells with EGF induces an activation and translocation of c-Src to the cytoskeleton. These processes depend upon the presence of the actin binding domain of the EGF-receptor since in cells that express EGF-receptors lacking this domain, EGF fails to induce an activation and translocation to the cytoskeleton of c-Src. These data suggest a role for the actin binding domain of the EGF-receptor in the translocation of c-Src.

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Citations

Dec 4, 2003·Biochemical and Biophysical Research Communications·Jun Tang, David J Gross
Jun 12, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ala-Eddin Al MoustafaMoulay A Alaoui-Jamali
Apr 9, 2008·Biochemical and Biophysical Research Communications·Wei SongQishui Lin
Jun 8, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vernella Vickerman, Roger D Kamm
Mar 9, 2007·FEMS Immunology and Medical Microbiology·Ignacio J JuncadellaJuan Anguita
Nov 17, 2001·The Journal of Biological Chemistry·John T SeykoraPaul L Stein
Jun 5, 2003·The Journal of Biological Chemistry·Zubin MasterDaniel J Dumont
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Boonstra

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