Epidermal growth factor-induced association of the SHPTP2 protein tyrosine phosphatase with a 115-kDa phosphotyrosine protein.

The Journal of Biological Chemistry
K Yamauchi, J E Pessin

Abstract

Epidermal growth factor (EGF) stimulation of HepG2 and NIH 3T3 cells expressing high levels of the human EGF receptor (3T3/ER) resulted in the tyrosine phosphorylation of a 115-kDa protein that was co-immunoprecipitated with the Src homology 2 (SH2) domain containing protein tyrosine phosphatase, SHPTP2. In contrast, activation of the EGF receptor resulted in a relatively low level (< 1%) of the total SHPTP2 pool associated with the tyrosine-autophosphorylated EGF receptor itself. Similarly, quantitative immunoprecipitations also demonstrated that only trace amounts of the total EGF receptor pool were associated with SHPTP2. Further, activation of the EGF receptor did not result in any significant tyrosine phosphorylation of SHPTP2 and/or the association of the 115-kDa protein with Grb2. In comparison, activation of Jurkat cells with a T cell receptor agonist monoclonal antibody resulted in the co-immunoprecipitation of a 120-kDa tyrosine-phosphorylated protein with Grb2 and a 105-kDa protein with SHPTP2. Thus, these data have identified the 115- and 105-kDa proteins as the predominant SHPTP2-associated phosphotyrosine proteins in EGF- and T cell receptor-activated cells, respectively.

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KoretzkyA Weiss
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·K YamauchiJ E Pessin
Feb 17, 1995·The Journal of Biological Chemistry·S SawasdikosolS J Burakoff
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A KazlauskasM Valius

❮ Previous
Next ❯

Citations

Jun 1, 1997·Current Opinion in Immunology·B G Neel
Apr 1, 1997·Current Opinion in Cell Biology·B G Neel, N K Tonks
Jul 1, 1997·European Journal of Biochemistry·K KrishnanJ J Krolewski
Dec 14, 1999·Molecular and Cellular Biology·A M O'ReillyB G Neel
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·Y Z Wang, J C Bonner
Apr 8, 1998·Current Opinion in Genetics & Development·D Van VactorB G Neel
Jan 10, 1997·The Journal of Biological Chemistry·G HuyerC Ramachandran
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y AokiS A Reeves
Jan 7, 1998·Molecular and Cellular Biology·A M O'Reilly, B G Neel
Jan 27, 1998·The Journal of Biological Chemistry·S O KimS J Frank
Jul 5, 2012·Molecular and Cellular Biochemistry·Zhenzhen HuXing Feng

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.