PMID: 6982901Oct 1, 1982Paper

Epidermal growth factor-induced centrosomal separation: mechanism and relationship to mitogenesis

The Journal of Cell Biology
P Sherline, R N Mascardo

Abstract

Using a rabbit antibody to MAP1 to stain centrosomes we have studied the mechanism by which epidermal growth factor (EGF) induces centrosomal separation in HeLa cells. The response is rapid, being detectable within 20 min after EGF (100 ng/ml) addition and by 4 h 40% of logarithmically growing cells and greater than 70% of cells synchronized at G1/S with 1 mM hydroxyurea show centrosomes separated by more than one diameter. A concentration of 0.05 ng/ml of EGF induces significant separation in synchronized cells (5-9% control vs. 20% with EGF at 0.05 ng/ml) and 0.1 to 0.5 ng/ml induces a half maximal response. Centrosomal separation is blocked by energy inhibitors, trifluoperazine, chlorpromazine, and W-7, cytochalasins B and D, and taxol, and is stimulated or enhanced by A23187, colchicine, and oncodazole. Trifluoperazine, W-7, cytochalasin D, and taxol also block DNA synthesis in response to EGF as measured by autoradiography using [3H]thymidine. Our hypothesis based upon these results is that EGF, by raising the free calcium level, activates calmodulin, which stimulates contraction of microfilaments attached to the centrosome, pulling the daughter centrosome apart. EGF may also induce depolymerization or detachment of microt...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A McKannaS Cohen
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S E Hutchings, G H Sato
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A M OttoL Jimenez de Asua
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M FriedkinE Rozengurt
Nov 1, 1978·The Journal of Cell Biology·J A Connolly, V I Kalnins
Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
Jun 1, 1977·The Journal of Cell Biology·R R Gould, G G Borisy
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F R Frankel
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Jun 1, 1976·Journal of Cellular Physiology·G Carpenter, S Cohen
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Rozengurt, L A Heppel
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Osborn, K Weber
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H A Armelin
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M D Hollenberg, P Cuatrecasas
May 1, 1973·The Journal of Cell Biology·J B Rattner, S G Phillips
Feb 1, 1968·The Journal of Cell Biology·E RobbinsA Micali
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
Jan 1, 1980·Annals of the New York Academy of Sciences·B WeissT L Wallace
Jan 1, 1980·Annual Review of Biochemistry·C B KleeP G Richman
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H HidakaT Nagata
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y MoroiE M Tan
May 1, 1982·The Journal of Cell Biology·P Sherline, R N Mascardo
May 8, 1980·Nature·A R Means, J R Dedman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A McClain, G M Edelman
Jan 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C WillinghamI Pastan
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Jimenez de AsuaA M Otto
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A M OttoL Jimenez de Asua
Nov 1, 1981·The Journal of Cell Biology·A I GotliebV I Kalnins
Dec 1, 1981·The Journal of Cell Biology·R Kuriyama, G G Borisy

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Citations

Jul 1, 1984·Experimental Cell Research·P Sherline, R N Mascardo
Jan 1, 1984·Pharmacology & Therapeutics·J J Manfredi, S B Horwitz
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G S BloomR B Vallee
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J S BonifacinoI V Sandoval
Apr 16, 2005·Molecular and Cellular Biology·Ratna K VadlamudiRakesh Kumar
Aug 1, 1984·The Journal of Clinical Investigation·P Sherline, R Mascardo
Oct 1, 1984·The Journal of Clinical Investigation·R N Mascardo, P Sherline
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fikret G AgircanBalca R Mardin
Oct 6, 2005·The Journal of Cell Biology·Susanne BaheErich A Nigg
Oct 1, 1984·Clinical Immunology and Immunopathology·R N MascardoP Sherline
Apr 29, 2014·Biochimica Et Biophysica Acta·Jing ZhaoBo Zhang
Dec 19, 2017·Frontiers in Cell and Developmental Biology·Andrew M FryJoan Roig
Sep 11, 2002·The Journal of Biological Chemistry·Masumi EtoDavid L Brautigan
Jan 1, 1986·Cell Motility and the Cytoskeleton·R KuriyamaG G Borisy
Jul 30, 2014·Transplantation·Jukka M RintalaEva von Willebrand
Aug 6, 2004·The Journal of Biological Chemistry·Brandon MetgeRon Balczon
Nov 29, 2007·Journal of Cell Science·Susanne GraserErich A Nigg
Jan 1, 1996·Blood Cells, Molecules & Diseases·S F PietromonacoL Elias
Nov 3, 1989·Science·A B Pardee

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