Epidermal growth factor-induced selective phosphorylation of cultured rat hepatocyte 55-kD cytokeratin before filament reorganization and DNA synthesis.

The Journal of Cell Biology
H BaribaultN Marceau

Abstract

We have reported previously that the addition of dexamethasone to cultured quiescent suckling rat hepatocytes in the presence of insulin, a culture condition which does not cause growth activation, induces a selective increase in the synthesis of the 49-kD/55-kD cytokeratin (CK49/CK55) pair over a 24-h period. This increased synthesis coincides with the formation of dense filament networks reminiscent of those observed in situ at the cell periphery (Marceau, N., H. Baribault, and I. Leroux-Nicollet. 1985. Can. J. Biochem. Cell Biol. 63:448-457). We show here for the first time that when EGF is added 48 h after insulin and dexamethasone, there is an early preferential phosphorylation of the CK55 of the CK49/CK55 pair, an induced filament rearrangement from the cell periphery to the cytoplasm, and a subsequent entry into S phase and mitosis after a lag period of 8 h. Indirect immunofluorescence microscopy with monoclonal antibodies to CK49 and CK55 indicate that, while before EGF treatment the cytokeratin filaments were mainly distributed near the cell periphery, the addition of EGF resulted in their reorganization to a predominantly cytoplasmic localization within less than 3 h. Antitubulin and anti-actin antibodies showed no de...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HaiglerS Cohen
Sep 1, 1985·Cell·P M SteinertD R Roop
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J K Franz, W W Franke
Aug 1, 1986·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·N MarceauH Gourdeau
May 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y KatsumaS W French
Dec 1, 1988·Experimental Cell Research·L DomenjoudA Alonso
May 1, 1986·Experimental Cell Research·G Carpenter, J G Zendegui
Jan 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E G Fey, S Penman
Jul 29, 1983·Biochemical and Biophysical Research Communications·I Leroux-NicolletN Marceau
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Fuchs, D Marchuk
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K H KimE Fuchs
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Aug 14, 1980·Biochemical and Biophysical Research Communications·S M Lastick, E H McConkey
May 1, 1981·The Journal of Clinical Investigation·H S Earp, E J O'Keefe

❮ Previous
Next ❯

Citations

May 26, 1999·Cell Motility and the Cytoskeleton·M I SatohJ E Coe
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman
Dec 1, 1996·Cancer Metastasis Reviews·N O KuM B Omary
Apr 17, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Geoffrey D YoungRobert I Garver
Feb 1, 1993·Current Opinion in Cell Biology·P A Coulombe
Jul 1, 1997·The International Journal of Biochemistry & Cell Biology·A WaseemN H Waseem
Jun 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Marcin MochReinhard Windoffer
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R W Donaldson, S Cohen
Jan 12, 2012·The Journal of Biological Chemistry·Byung-Min ChungPierre A Coulombe
May 1, 1992·The Journal of Cell Biology·M B OmaryB Strulovici
Nov 1, 1992·The Journal of Cell Biology·J A DentM W Klymkowsky
Aug 1, 1996·The Journal of Cell Biology·R D GoldmanP M Steinert
Sep 1, 1996·Clinical and Experimental Pharmacology & Physiology·V TkachukA Bobik
Nov 21, 1992·Annals of the New York Academy of Sciences·G Bellomo, F Mirabelli
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·Y KohnoH Akiyoshi
Jan 25, 2003·The Journal of Investigative Dermatology·Pavel StrnadRudolf E Leube
May 15, 2007·Experimental Cell Research·Normand MarceauFrans C S Ramaekers
Nov 28, 2006·Experimental Cell Research·Anne LorangerNormand Marceau
Nov 23, 2006·Experimental Cell Research·Luc GalarneauNormand Marceau
Aug 31, 1990·Biochemical and Biophysical Research Communications·M Mesarić, K Decker
Jul 28, 2013·Current Opinion in Cell Biology·Byung-Min ChungPierre A Coulombe
Jan 16, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A MeyerdierksE C Böttger
Sep 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Cadrin, M G Martinoli
Sep 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A LorangerN Marceau
Jan 10, 2002·The Journal of Biological Chemistry·Tao HeJohn E Eriksson
Jan 1, 1990·Life Sciences·H KawaharaS W French
Oct 1, 1992·Trends in Cell Biology·O SkalliR D Goldman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.