PMID: 8581878Dec 1, 1995Paper

Epidermal growth factor induces Egr-1 messenger RNA and protein in mouse osteoblastic cells

Calcified Tissue International
M A FangS McDougall

Abstract

The nuclear signaling events activated when epidermal growth factor (EGF) interacts with osteoblasts to produce effects on growth and differentiation are not clearly understood, and may include induction of immediate early genes such as Egr-1, a zinc finger transcription factor. In the present study, Northern analyses were performed to define the effects of EGF on the expression of Egr-1 mRNA in MC3T3-E1 mouse osteoblastic cells. Following treatment of quiescent, subconfluent MC3T3-E1 cells with 0.1-100 ng/ml EGF for various periods, maximal induction of Egr-1 mRNA occurred when cells were treated for 30-60 minutes with 1-10 ng/ml EGF. Inhibition of protein kinase C activity by pretreatment with 1 microM chelerythrine chloride or by prolonged stimulation with 50 ng/ml tetradecanoyl phorbol acetate (TPA) partially diminished the induction of Egr-1 by EGF. Using an immunohistochemical approach, 10 ng/ml EGF was observed to induce Egr-1 protein within 30-60 minutes and this induction was localized to the nucleus. These observations indicate that EGF induces Egr-1 mRNA and protein via protein kinase C and other signaling pathways, and that Egr-1 may be part of the regulatory network mediating the actions of EGF on growth and differ...Continue Reading

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Citations

Oct 25, 2000·Human Gene Therapy·M BryantM Braddock
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M R MoalliS A Goldstein
Jan 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jason P HattonMillie Hughes-Fulford
May 1, 2001·Biochemical and Biophysical Research Communications·P HoustonM Braddock
Jun 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chai-Fei Li, Millie Hughes-Fulford
Jan 3, 2013·Journal of Tissue Engineering and Regenerative Medicine·Kyung Shin KangDong-Woo Cho
Feb 15, 2001·Oncogene·A Raouf, A Seth
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J C TsaiW C Aird
Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J C TsaiW C Aird

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