Epidermal growth factor initiates DNA synthesis after a time-dependent sequence of regulatory events in Swiss 3T3 cells--interactions with hormones and growth factors

Journal of Cellular Physiology
A M OttoL Jimenez de Asua

Abstract

Epidermal growth factor (EGF) stimulates the initiation of DNA synthesis in Swiss 3T3 cells after a constant prereplicative period of 14-15 hours. The final rate of initiation follows apparent first-order kinetics and can thus be quantified by a rate constant k. The value of k can be changed by later additions during the prereplicative period: When cells stimulated by a very low concentration of EGF, alone or with insulin, which results in a relatively low value of k, receive a saturating amount of EGF at 15 hours, then k is markedly increased after 4-6 hours. Insulin alone (up to 200 ng/ml) is unable to set the lag phase, but does have a synergistic effect on the value of k given by EGF. When added at 15 hours, insulin also increases K, but after a delay of 4-6 hours. In contrast, both hydrocortisone and prostaglandin E1 (PGE1) inhibit the stimulation of DNA synthesis by EGF only during the first 8 hours of the prereplicative period of decreasing the value of k. Prostaglandin F2 alpha), which stimulates DNA synthesis in a similar mode as EGF, when added with EGF has a synergistic effect on DNA synthesis. This suggests that EGF and PGF2 alpha, nevertheless, act through different regulatory events.

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Citations

Jan 1, 1993·Cell Proliferation·J E Souren, R van Wijk
Apr 1, 1985·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·R van WijkL Jimenez de Asua
Nov 1, 1985·Journal of Clinical Pharmacology·N CharletR J Wills
Nov 1, 1986·Experimental Cell Research·D VaulotS W Chisholm
Jul 28, 1986·FEBS Letters·C BlatL Harel
May 1, 1984·Journal of Cellular Physiology·R van WijkL Jimenez de Asua
Jul 28, 2019·Virus Research·Guohong CaiBradley I Hillman
Dec 1, 1983·Experimental Cell Research·S Talha, L Harel
Jan 1, 1983·Journal of Cellular Biochemistry·C L MummeryS W de Laat

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