Epidermal growth factor-like motifs 1 and 2 of Plasmodium vivax merozoite surface protein 1 are critical domains in erythrocyte invasion

Biochemical and Biophysical Research Communications
Hye-Jin HanJoon-Yong Chung

Abstract

Plasmodium vivax merozoite surface protein 1 (PvMSP1) is believed to be important in erythrocyte invasion. However, the detailed mechanism of PvMSP1-mediated invasion has been unclear. We demonstrate that the C-terminal 19 kDa domain (PvMSP119) of PvMSP1, the 42-kDa fragment of PvMSP1 is further cleaved to a 33 kDa N-terminal polypeptide and a 19 kDa C-terminal fragment in a secondary processing step, is a critical domain in the binding between parasite ligand and erythrocyte receptor. Also, its cytoadherence was successfully blocked by naturally acquired immunity, was partially sensitive to neuraminidase and trypsin. When expressed separately epidermal growth factor (EGF)-like motifs 1 and 2, subunits of the PvMSP119, mediated 64% and 66% of the erythrocyte-binding activity, respectively, relative to their expression together as a single intact ligand domain. These results suggest that the EGF-like motifs 1 and 2 of PvMSP119 function as a core-binding portion in the attachment of PvMSP1 to erythrocytes.

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Citations

Mar 17, 2011·PloS One·Philipp LudinPascal Mäser
Sep 20, 2007·Proteins·Faraz Alam AnsariSrinivasan Ramachandran
May 28, 2014·The Journal of Infectious Diseases·Rajesh ChandramohanadasPeter Preiser
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Oct 3, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M Andreina PachecoAnanias A Escalante
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Apr 27, 2007·Clinical and Vaccine Immunology : CVI·So-Hee KimWeon-Gyu Kho
Mar 1, 2017·Frontiers in Immunology·Carolina LópezManuel A Patarroyo
Jan 23, 2021·Expert Review of Vaccines·Sai Lata DeJohn H Adams
Feb 11, 2021·International Journal of Molecular Sciences·Laura Alejandra Ricaurte-ContrerasManuel Alfonso Patarroyo
Oct 16, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Natália Ketrin Almeida-de-OliveiraMaria de Fátima Ferreira-da-Cruz

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