Epidermal growth factor: modulator of murine embryonic palate mesenchymal cell proliferation, polyamine biosynthesis, and polyamine transport

Journal of Cellular Physiology
K Gawel-Thompson, R M Greene

Abstract

Polyamines (putrescine, spermidine, and spermine) are normal cellular constituents able to modulate cellular proliferation and differentiation in a number of tissues and cell types. This investigation explores the response of murine embryonic palate mesenchymal (MEPM) cells to epidermal growth factor (EGF) in terms of biosynthesis of putrescine and its transport across the plasma membrane and tests the hypothesis that polyamine transport can serve as an alternative mechanism (other than biosynthesis) for elevating intracellular polyamines during stimulation of MEPM cellular proliferation. MEPM cells treated with EGF were stimulated to proliferate and showed a dose- and time-dependent stimulation of ornithine decarboxylase (ODC) which was maximal at 4-6 hours. EGF also stimulated the initial rate of putrescine transport in a dose- and time-dependent manner. This stimulation was found to be maximal 3 hours after treatment and specific for the putrescine transport system. The kinetic parameters of putrescine transport shifted from 2.52 microM (Km) and 23.6 nmol/mg protein/15 minutes (Vmax) in nonstimulated cells to 4.48 microM (Km) and 39.8 nmol/mg protein/15 minutes (Vmax) in EGF-treated cells. This kinetic shift did not require ...Continue Reading

References

Jan 1, 1977·Journal of Supramolecular Structure·M L Vilereal, J S Cook
Jan 1, 1987·Current Topics in Developmental Biology·R M Pratt
Jan 1, 1986·Journal of Cellular Physiology·M M PisanoR M Greene
Aug 1, 1988·Journal of Cellular Physiology·K Gawel-Thompson, R M Greene
May 3, 1988·Biochemistry·Y Yarden, A Ullrich
Dec 1, 1974·Archives of Biochemistry and Biophysics·A Lajtha, H Sershen
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Russell, S H Snyder
Jan 31, 1972·Biochimica Et Biophysica Acta·D H Russel, T A McVicker
Apr 30, 1981·Biochemical and Biophysical Research Communications·D H Russell
Jan 1, 1983·Methods in Enzymology·T A Slotkin, J Bartolome
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E NexøR M Pratt
Jul 16, 1981·Biochemical and Biophysical Research Communications·K Y Chen, C A Rinehart
Dec 1, 1981·Journal of Cellular Physiology·D R Bethell, A E Pegg
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
May 1, 1981·Journal of Cellular Physiology·J S Heller, E S Canellakis
Oct 1, 1980·Journal of Cellular Physiology·A F BorghettiG G Guidotti

❮ Previous
Next ❯

Citations

Aug 1, 1996·The International Journal of Biochemistry & Cell Biology·N SeilerJ P Moulinoux
May 26, 1998·Annals of the New York Academy of Sciences·R M Lafrenie, K M Yamada
Jun 15, 2007·Critical Reviews in Clinical Laboratory Sciences·Nikolaus Seiler, Francis Raul
Jun 1, 1990·Journal of Cellular Physiology·T L Byers, A E Pegg
Apr 1, 1992·The Journal of Experimental Zoology·K J Gawel-Thompson, R M Greene
Oct 1, 1991·Kidney International·E Schulze-LohoffR B Sterzel
Sep 6, 2005·Differentiation; Research in Biological Diversity·Ildikó LoikkanenSeppo Vainio
Jul 2, 1998·Journal of Cellular Physiology·M B PotchinskyR M Greene
Sep 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S Nagoshi, K Fujiwara
Jan 27, 1995·The Journal of Biological Chemistry·M LessardR Poulin
Mar 6, 2015·Journal of Biosciences·Indraneel MittraAmit Dutt
May 1, 1991·Molecular and Cellular Endocrinology·G Scalabrino, E C Lorenzini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.