Epidermal growth factor receptor (EGFR) density may not be the only determinant for the efficacy of EGFR-targeted photoimmunotherapy in human head and neck cancer cell lines

Lasers in Surgery and Medicine
Wei PengDominic J Robinson

Abstract

The aim of this study was to investigate the effects of targeted photoimmunotherapy (PIT) in vitro on cell lines with various expression levels of epidermal growth factor receptor (EGFR) using an anti-EGFR targeted conjugate composed of Cetuximab and IR700DX, phthalocyanine dye. Relative EGFR density and cell binding assay was conducted in three human head & neck cancer cell lines (scc-U2, scc-U8, and OSC19) and one reference cell line A431. After incubation with the conjugate for 1 or 24 hours, cellular uptake and localization were investigated by confocal laser scanning microscopy and quantified by image analysis. Cell survival was determined using the MTS assay and alamarBlue assay after PIT with a 690 nm laser to a dose of 7 J.cm-2 (at 5 mW.cm-2 ). The mode of cell death was examined with flow cytometry using apoptosis/necrosis staining by Annexin V/propidium iodide, together with immunoblots of anti-apoptotic Bcl-2 family proteins Bcl-2 and Bcl-xL. A431 cells had the highest EGFR density followed by OSC19, and then scc-U2 and scc-U8. The conjugates were localized both on the surface and in the cytosol of the cells after 1- and 24-hour incubation. After 24-hour incubation the granular pattern was more pronounced and in a si...Continue Reading

References

Jan 1, 1989·Seminars in Surgical Oncology·T J Dougherty
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Nov 1, 1996·Photochemistry and Photobiology·J HeN L Oleinick
Dec 2, 2000·Journal of Photochemistry and Photobiology. B, Biology·A C Moor
Sep 8, 2001·Photochemistry and Photobiology·D Kessel, M Castelli
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nancy L OleinickIrina Belichenko
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·David Kessel
May 6, 2003·Biochemical and Biophysical Research Communications·Guido Kroemer
Jul 15, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R HamidP Bullock
Jun 24, 2006·Nature Reviews. Cancer·Ana P CastanoMichael R Hamblin
Mar 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jun KuraiEiji Shimizu
Oct 16, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cristianne J F RijckenCornelus F van Nostrum
Jan 22, 2008·Addictive Behaviors·Robert ScraggJoseph R DiFranza
Jul 4, 2008·Pharmaceutical Research·Jan-Willem HofmanCornelus F van Nostrum
Dec 19, 2009·Cell Death and Differentiation·I K H PoonC R Parish
May 17, 2011·Current Medicinal Chemistry·A Casas Al Batlle
Nov 29, 2013·Photodiagnosis and Photodynamic Therapy·Ron R Allison, Keyvan Moghissi
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Jan 8, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Raimond HeukersSabrina Oliveira

❮ Previous
Next ❯

Citations

Feb 23, 2020·Journal of Clinical Medicine·Waseem JerjesKristian Berg
Jul 18, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lulu HanLingyun Jia
Apr 25, 2020·Cancers·Irati Beltrán HernándezSabrina Oliveira
Apr 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marion M DekenSabrina Oliveira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.