Epidermal growth factor receptor is critical for angiotensin II-mediated hypertrophy in cerebral arterioles

Hypertension
Siu-Lung ChanGary L Baumbach

Abstract

Angiotensin II (Ang II) is a major determinant of vascular remodeling in the cerebral circulation during chronic hypertension, which is an important risk factor for stroke. We examined the molecular mechanism of Ang II-mediated cerebrovascular remodeling that involves the epidermal growth factor receptor (EGFR) pathway. Mutant EGFR mice (waved-2), their heterozygous control (wild-type [WT]), and C57BL/6J mice were infused with Ang II (1000 ng kg(-1) min(-1)) or saline via osmotic minipumps for 28 days (n=8 per group). Eight of the Ang II-infused C57BL/6J mice were cotreated with AG1478 (12 mg/kg per day, IP), a specific EGFR tyrosine kinase inhibitor. Systolic arterial pressure was measured by a tail-cuff method. Pressure and diameter of cerebral arterioles were measured through an open cranial window in anesthetized mice. Cross-sectional area of the wall was determined in pressurized fixed cerebral arterioles. Expression of phosphorylated EGFR (p-EGFR), caveolin-1 (Cav-1), and c-Src was determined by western blotting and immunohistochemistry. Mutation of EGFR or AG1478 treatment did not affect Ang II-induced hypertension. Ang II increased the expression of p-EGFR in WT mice, confirming the activation of EGFR. Ang II induced hy...Continue Reading

References

Jun 1, 1989·Hypertension·G L Baumbach, D D Heistad
Apr 17, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M Ushio-FukaiR W Alexander
Jan 4, 2003·Hypertension·Gary L BaumbachFrank M Faraci
Jan 22, 2005·Journal of Hypertension·Ashley S IzzardAnthony M Heagerty
Apr 20, 2005·Journal of Cardiovascular Pharmacology·Pierre Beaucage, Pierre Moreau
Jun 3, 2006·American Journal of Respiratory and Critical Care Medicine·Yoshiki IshiiTakeshi Fukuda
Jun 28, 2013·Frontiers in Physiology·Siu-Lung Chan, Gary L Baumbach

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Citations

Nov 14, 2015·Annual Review of Pharmacology and Toxicology·Steven J ForresterSatoru Eguchi
Mar 26, 2016·Frontiers in Pharmacology·T Michael De Silva, Alyson A Miller
Mar 19, 2016·Cellular and Molecular Neurobiology·T Michael De Silva, Frank M Faraci
Aug 9, 2016·European Journal of Pharmacology·Melissa SkibbaGuang Liang
Jun 7, 2018·Physiological Reviews·Steven J ForresterSatoru Eguchi
Sep 11, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marilyn J CipollaSiu-Lung Chan
Feb 6, 2017·Circulation Research·Xiaoming HuFrank M Faraci
Dec 16, 2016·Hypertension·Costantino IadecolaUNKNOWN American Heart Association Council on Hypertension; Council on Clinical Cardiology; Council on Cardiovascular Disease in the
Feb 13, 2020·International Journal of Molecular Sciences·Rita Moretti, Paola Caruso
Jul 1, 2016·Physiological Reviews·Jianchun ChenRaymond C Harris
Jan 17, 2020·CNS Neuroscience & Therapeutics·Cheng-Cheng LiHua Feng
Aug 20, 2019·Postgraduate Medicine·Pitchaya WanleenuwatWojciech Kozubski
Nov 24, 2020·British Journal of Pharmacology·Yuhong JiangDavid P Fairlie

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