PMID: 9529375May 16, 1998Paper

Epidermal growth factor signaling and mitogenesis in Plcg1 null mouse embryonic fibroblasts

Molecular Biology of the Cell
Q s JiGraham Carpenter

Abstract

Gene targeting techniques and early mouse embryos have been used to produce immortalized fibroblasts genetically deficient in phospholipase C (PLC)-gamma1, a ubiquitous tyrosine kinase substrate. Plcg1(-/-) embryos die at embryonic day 9; however, cells derived from these embryos proliferate as well as cells from Plcg1(+/+) embryos. The null cells do grow to a higher saturation density in serum-containing media, as their capacity to spread out is decreased compared with that of wild-type cells. In terms of epidermal growth factor receptor activation and internalization, or growth factor induction of mitogen-activated protein kinase, c-fos, or DNA synthesis in quiescent cells, PLcg1(-/-) cells respond equivalently to PLcg1(+/+) cells. Also, null cells are able to migrate effectively in a wounded monolayer. Therefore, immortalized fibroblasts do not require PLC-gamma1 for many responses to growth factors.

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Citations

Aug 5, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Carpenter
Oct 8, 1999·Molecular and Biochemical Parasitology·C M OchattG A Cross
Apr 21, 1999·Current Opinion in Cell Biology·N Moghal, P W Sternberg
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Jan 20, 2005·Journal of Cell Science·Denis TvorogovGraham Carpenter
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