PMID: 1461718Aug 1, 1992

Epidermal growth factor stimulates Ca2+ uptake of human erythrocytes

Pflügers Archiv : European journal of physiology
B EngelmannJ Duhm

Abstract

To examine the functional significance of epidermal growth factor (EGF) binding sites present on the human erythrocyte membrane [Engelmann et al. (1992) Am J Hematol 39:239-241], the effect of EGF on 45Ca2+ uptake and on 22Na+ efflux from these cells has been studied. In all cases media contained 1.25 mM Ca2+, whereas Na+ and K+ were varied. In 140 mM Na+/5 mM K+ medium EGF (250 ng/ml) stimulated 45Ca2+ uptake by 50%-90% in quin-2-loaded cells, and by up to threefold in untreated cells. Increasing extracellular K+ up to 75 mM at the expense of extracellular Na2+ stimulated the EGF-induced 45Ca2+ uptake by about twofold compared to 145 mM Na+ medium both in quin-2-loaded and in untreated cells. In 145 mM K+ medium, however, no EGF-induced 45Ca2+ uptake was detectable in quin-2-loaded cells, while in untreated cells Ca2+ entry was stimulated twofold by EGF. After increasing intracellular Na+ from 6 mmol/l cells to 18 mmol/l cells in untreated cells suspended in 145 mM K+ medium, 45Ca2+ uptake induced by EGF gradually increased. In contrast, in 140 mM Na+/5 mM K+ as well as in 70 mM Na+/75 mM K+ medium, 45Ca2+ uptake accelerated by EGF was largely unaffected by a modified red cell Na+ content. When 22Na-loaded untreated red cells ...Continue Reading

References

Jun 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·B Engelmann, U Schumacher
Mar 9, 2004·Biochimica Et Biophysica Acta·Roman HudecL Varecka
Nov 24, 2004·Biochemical and Biophysical Research Communications·Roman HudecL Varecka
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Philipp AttanasioFlorian Lang

Related Concepts

Calcium Radioisotopes
Quin2
Epidermal Growth Factor
Calcium
Ouabain
Sodium Radioisotopes
Sodium
Aminoquinolines
Ion Exchange
Erythrocytes

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