PMID: 8962701Nov 1, 1995Paper

Epidermal growth factor stimulates the restitution of rat gastric mucosa in vitro

Experimental Physiology
C A Miller, H T Debas

Abstract

Epidermal growth factor (EGF) is mitogenic to gastric mucosal cells, protects against gastric mucosal injury induced by ulcerogenic agents, and stimulates cell migration in many systems. Restitution, the rapid re-establishment of mucosal integrity following damage, involves epithelial cell migration and can be monitored by measuring the transmucosal potential difference (PD) of tissue mounted in an Ussing chamber. The role of EGF in restitution is poorly characterized. To assess the effect of exogenous EGF on restitution, samples of rat gastric mucosa were mounted in an Ussing chamber with a solution of 10 nM EGF bathing either the mucosal or serosal surface. Matched control tissues from the same animals were untreated. Mucosal damage was induced by exposure of tissues to hyperosmolar sodium chloride. Restitution following injury was monitored by measuring the recovery of transmucosal PD, which reflects the degree of mucosal integrity. Undamaged mucosa maintained a PD of approximately -30 mV for several hours (mucosa negative with respect to serosa). Mucosal exposure to 1.2 M NaCl for 2 min reduced the PD to near 0 mV. Thereafter, the PD returned in approximately 60 min to plateau at a value representing maximal recovery. Tissu...Continue Reading

Citations

Apr 25, 2013·Seminars in Pediatric Surgery·Kathryn J RowlandBrad W Warner
Aug 9, 2005·Seminars in Pediatric Surgery·Brad W Warner, Barbara B Warner
Jul 20, 2002·The Journal of Surgical Research·Carlos AlvarezBarbara L Bass
Dec 22, 2006·Cell Motility and the Cytoskeleton·Ramesh M RayLeonard R Johnson
Oct 22, 2004·American Journal of Physiology. Cell Physiology·Rajiv J VaidyaLeonard R Johnson
Nov 2, 2019·Physiological Reviews·Amy C EngevikJames R Goldenring
Mar 9, 2010·Journal of Biomolecular Screening·Patrick Y K YueRicky N S Wong
Apr 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akinori YanakaNaomi Tanaka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.