Epidermal morphogenesis: the transcriptional program of human keratinocytes during stratification

The Journal of Investigative Dermatology
Piyush Koria, Stelios T Andreadis

Abstract

The epidermis serves to protect the body against environmental assaults and at the same time is able to survive and replenish itself under harsh conditions. The epidermis accomplishes this feat via a well-orchestrated program of stratification and terminal differentiation that provides barrier against infection, radiation, and water loss. Despite significant progress in skin biology, many molecules and pathways that are involved in stratification and barrier formation remain unknown. Here, we employed tissue-engineered models of complete versus impaired epidermal stratification to discover the genes that may be important in this process. Transcriptional profiling at different stages of development showed significant differences in transcription, signaling, and most important metabolism-associated genes between fully stratified and poorly stratified epithelia. These transcriptional changes correlated well with functional data on cell proliferation, expression of adhesion molecules, and utilization of metabolic pathways, ultimately leading to different phenotypes. Our data identified genes that were not previously known to play a role in epidermis and established a link between metabolism and morphogenesis in skin epithelium.

References

Jan 1, 1987·Journal of Cell Science. Supplement·F GrinnellA Takashima
Aug 1, 1989·The Journal of Dermatology·T ShimouraK Aso
Jan 1, 1974·Journal of Surgical Oncology·J E Hoopes, M J Im
Sep 1, 1972·The Journal of Investigative Dermatology·A OhkawaraV Levine
Jul 1, 1983·The Journal of Investigative Dermatology·M PruniérasD Woodley
Sep 1, 1984·Clinical Science·C S Harmon, P J Phizackerley
Jul 1, 1984·The British Journal of Dermatology·D Asselineau, M Prunieras
Sep 1, 1993·The Journal of Clinical Investigation·H LarjavaJ Heino
Jul 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G J Fisher, J J Voorhees
May 7, 1999·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·S D DimitrijevichJ G Mills
May 18, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·K C OngenaeH Y Park
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S T AndreadisJ R Morgan
Apr 25, 2002·Nature Reviews. Genetics·Elaine Fuchs, Srikala Raghavan
Dec 3, 2002·Tissue Engineering·David J GeerStelios T Andreadis
Jul 26, 2003·The Journal of Investigative Dermatology·Piyush KoriaStelios T Andreadis
Nov 25, 2003·The Journal of Investigative Dermatology·Isabella SaviniLuciana Avigliano
Jan 8, 2004·The Journal of Investigative Dermatology·David J Geer, Stelios T Andreadis
Jul 24, 2004·The International Journal of Developmental Biology·Daniel Aberdam
Sep 15, 2004·Tissue Engineering·David J GeerStelios T Andreadis

❮ Previous
Next ❯

Citations

Oct 31, 2006·Archives of Dermatological Research·Yves Poumay, Alain Coquette
Oct 23, 2008·Annals of Biomedical Engineering·Raghvendra SinghStelios T Andreadis
Aug 12, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meng-Horng LeeStelios T Andreadis
Oct 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meng-Horng LeeStelios T Andreadis
Oct 20, 2006·Expert Opinion on Biological Therapy·Stelios T Andreadis
Jan 26, 2008·The Journal of Investigative Dermatology·Melanie SchüppelBurkhard Kleuser
Nov 23, 2012·Clinical and Experimental Dermatology·T Tempark, T Shwayder
Jul 31, 2013·Journal of Tissue Engineering and Regenerative Medicine·Ruchi SharmaF Xavier Donadeu
Jul 3, 2016·Colloids and Surfaces. B, Biointerfaces·Simona SalernoLoredana De Bartolo
Sep 24, 2016·The American Journal of Dermatopathology·Sangjun LeeTimothy McDonnell
Aug 11, 2012·Annals of Dermatology·Seung Ju BackJeung Hoon Lee
Feb 15, 2011·The American Journal of Dermatopathology·Juan García-GavínJaime Toribio
Jun 29, 2007·American Journal of Physiology. Cell Physiology·Piyush Koria, Stelios T Andreadis
Nov 21, 2012·Experimental Dermatology·Aurélie FrankartYves Poumay
Nov 12, 2020·Scientific Reports·Amanda C Camillo-AndradePaulo C Carvalho
Apr 18, 2013·Journal of Cell Science·Hui YouStelios T Andreadis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved