Jul 25, 2015

Epigallocatechin-3-gallate enhances clearance of phosphorylated tau in primary neurons

Nutritional Neuroscience
Adrianne S ChesserGail V W Johnson

Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by intracellular accumulations of phosphorylated forms of the microtubule binding protein tau. This study aimed to explore a novel mechanism for enhancing the clearance of these pathological tau species using the green tea flavonoid epigallocatechin-3-gallate (EGCG). EGCG is a potent antioxidant and an activator of the Nrf2 transcriptional pathway. Nrf2 activators including EGCG have shown promise in mitigating amyloid pathology in vitro and in vivo. This study assessed whether EGCG could also alter tau clearance. Rat primary cortical neuron cultures were treated on day in vitro 8 with EGCG and analyzed for changes in gene and protein expression using luciferase assay, q-PCR, and western blotting. EGCG treatment led to a significant decrease in the protein levels of three AD-relevant phospho-tau epitopes. Unexpectedly, EGCG does not appear to be facilitating this effect through the Nrf2 pathway or by increasing autophagy in general. However, EGCG did significantly increase mRNA expression of the key autophagy adaptor proteins NDP52 and p62. In this study, we show that EGCG enhances the clearance of AD-relevant phosphorylated tau species in primary neurons. I...Continue Reading

  • References63
  • Citations8

References

Mentioned in this Paper

Study
In Vivo
Biochemical Pathway
APP protein, human
NF-E2-Related Factor 2
Genes
Green Tea Extract
Western Blotting
Protoplasm
Transcription, Genetic

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