Epigallocatechin-3-gallate Inhibits LPS-Induced NF-κB and MAPK Signaling Pathways in Bone Marrow-Derived Macrophages.

Gut and Liver
So-Young JooYoung-Eun Joo

Abstract

Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of the current study was to characterize the impact of EGCG on lipopolysaccharide (LPS)-induced innate signaling in bone marrow-derived macrophages (BMMs) isolated from ICR mice. The effect of EGCG on LPS-induced pro-inflammatory gene expression and nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling was examined using reverse transcription-polymerase chain reaction, Western blotting, immunofluorescence, and the electrophoretic mobility shift assay. EGCG inhibited accumulation of LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs. EGCG blocked LPS-induced IκBα degradation and RelA nuclear translocation. EGCG blocked the DNA-binding activity of NF-κB. LPS-induced phosphorylation of ERK1/2, JNK, and p38 was inhibited by EGCG. U0126 (an inhibitor of MEK-1/2) suppressed the LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA accumulation in BMMs. These results indicate that EGCG may prevent LPS-induced pro-inflammatory gene expression through blocking NF-κB and MAPK signaling pathways in BMMs.

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Citations

Oct 23, 2015·International Journal of Molecular Sciences·Jun WangYushun Zhang
May 23, 2014·Experimental Gerontology·Thulasi Raman KrishnanKalaiselvi Periandavan
Jul 16, 2015·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Alexander Zink, Claudia Traidl-Hoffmann
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Feb 27, 2021·Critical Reviews in Food Science and Nutrition·Yina HuangHua Wei
Jul 7, 2021·Journal of Agricultural and Food Chemistry·Yaozhong HuShuo Wang
Nov 18, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Ayda CherianSreejith Madhavankutty

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Methods Mentioned

BETA
Assay
protein assay
PCR
nuclear translocation

Software Mentioned

Magellan V6

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