Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Frontiers in Pharmacology
Haihua ZhangGuangyu Li

Abstract

Background: Hair follicles play an essential role in the growth of hair. Epigallocatechin-3-gallate (EGCG), a catechin polyphenol in green tea, has various bioactivities. The present study aims to evaluate the effect of EGCG on the growth of mink hair follicles and investigate the possible molecular mechanisms. Methods: The length of hair follicles was recorded up to 6 days in presence of 0.1-5 μM EGCG. Primary dermal papilla cells (DPCs) and outer root sheath cells (ORSCs) were treated with 0.25-4 μM EGCG, and their growth was evaluated by MTT assay and cell cycle detection. The levels of key molecules in sonic hedgehog (Shh) and protein kinase B (AKT) signaling pathways were further assessed by quantitative real-time PCR, western blot and immunofluorescence. To determine the involvement of Shh and AKT pathways in EGCG-mediated growth-promotion of ORSCs and DPCs, Shh pathway inhibitors cyclopamine and GANT61 or AKT pathway inhibitor LY294002 were employed, and then cell proliferation and cell cycle were analyzed. Results: Data from ex vivo culture showed that, in presence of 0.5-2.5 μM EGCG, the growth of mink hair follicles was promoted. In vitro, the proliferation of DPCs and ORSCs was enhanced by 0.5-4 μM EGCG treatment. Mo...Continue Reading

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Citations

Aug 26, 2018·International Journal of Cosmetic Science·Alka MadaanManu Jaggi
Jul 31, 2020·Dose-response : a Publication of International Hormesis Society·Edward J CalabreseVittorio Calabrese
Dec 29, 2018·Journal of Experimental & Clinical Cancer Research : CR·Stefania RossiFrancesco Facchiano

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Methods Mentioned

BETA
PCR
protein assay
flow cytometry
nuclear translocation

Software Mentioned

CellSens Standard

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