Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway

BioMed Research International
Wang NanXu Zhongxin

Abstract

Epigallocatechin-3-gallate (EGCG) has neuroprotective effects and the ability to resist amyloidosis. This study observed the protective effect of EGCG against neuronal injury in rat models of middle cerebral artery occlusion (MCAO) and investigated the mechanism of action of PI3K/AKT/eNOS signaling pathway. Rat models of permanent MCAO were established using the suture method. Rat behavior was measured using neurological deficit score. Pathology and apoptosis were measured using HE staining and TUNEL. Oxidative stress and brain injury markers were examined using ELISA. Apoptosis-related proteins and PI3K/AKT/eNOS signaling pathway were determined using western blot assay and immunohistochemistry. EGCG decreased neurological function score, protected nerve cells, inhibited neuronal apoptosis, and inhibited oxidative stress injury and brain injury markers level after MCAO. EGCG reduced the apoptotic rate of neurons, increased the expression of Bcl-2, and decreased the expression of Caspase-3 and Bax. After LY294002 suppressed the PI3K pathway, the protective effect of EGCG decreased after administration of PI3K inhibitors. EGCG has a protective effect on rat brain injury induced by MCAO, possibly by modulating the PI3K/AKT/eNOS s...Continue Reading

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Citations

Dec 27, 2018·Nutrients·Naghma Khan, Hasan Mukhtar
Jan 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mehdi Sharifi-RadJavad Sharifi-Rad
Apr 5, 2020·Neural Regeneration Research·Tahereh FarkhondehSaeed Samarghandian
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May 20, 2021·Oxidative Medicine and Cellular Longevity·Hui XuMingfu Wang
Jul 3, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Abdulloh MachinDjoko A Purwanto
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Aug 31, 2021·Frontiers in Aging Neuroscience·Min-Huang HsiehShin-Da Lee

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Methods Mentioned

BETA
Enzyme Linked Immunosorbent Assay
MDA
electrophoresis

Software Mentioned

Image J
SPSS

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