Epigallocatechin Gallate Attenuates Bladder Dysfunction via Suppression of Oxidative Stress in a Rat Model of Partial Bladder Outlet Obstruction

Oxidative Medicine and Cellular Longevity
Meng GuZhong Wang

Abstract

To investigate the protective effect of epigallocatechin gallate (EGCG), a green tea extract, and its underlying mechanism on bladder dysfunction in a rat model of bladder outlet obstruction (BOO). Sprague-Dawley rats of BOO were surgically induced and followed by treatment with EGCG (5 mg/kg/day) or saline (control) via intraperitoneal injection. Cystometry was performed on four weeks postoperatively in conscious rats. H&E, Masson trichrome, and TUNEL staining were performed to observe tissue alterations. Oxidative stress markers were measured, and protein expression of Nrf2-ARE pathway was examined by immunohistochemistry and Western blotting. Our data showed that EGCG could increase the peak voiding pressure and bladder compliance and prolong micturition interval of BOO rats compared with control and finally reduce the frequency of urinary. EGCG could ameliorate the increase of collagen fibers and ROS induced by obstruction and increase the activity of SOD, GSH-Px, and CAT. The level of cell apoptosis was decreased in BOO rats treated with EGCG compared with control, and caspase-3 expression was reduced as well. Moreover, EGCG could activate the Nrf2 expression with elevation of its target antioxidant proteins. EGCG alleviat...Continue Reading

References

Dec 8, 2004·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·U UllmannP Weber
Jan 3, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R A IsbruckerJ Bausch
Mar 2, 2006·BJU International·John M Fitzpatrick
Aug 19, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kevin C Kregel, Hannah J Zhang
May 10, 2007·Neurourology and Urodynamics·Ahmet GuvenAnita Mannikarottu
May 25, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Pedro J Gómez-PinillaPedro J Camello
Mar 1, 2011·Experimental and Molecular Pathology·Joan OlivaSamuel W French
Mar 15, 2012·Neurourology and Urodynamics·Matthias OelkeJohn Heesakkers
Oct 24, 2014·International Journal of Urology : Official Journal of the Japanese Urological Association·Masanori NomiyaOsamu Yamaguchi
Dec 17, 2014·Urologia Internationalis·Paola Lucia MinciulloSebastiano Gangemi
Nov 12, 2015·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Bo ZhangYongqiang Wang

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Citations

Feb 25, 2020·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Guo ChenKunjie Wang
Nov 14, 2019·Antioxidants·Solomon Habtemariam
Jul 19, 2019·Oxidative Medicine and Cellular Longevity·Chong LiuZhong Wang
Feb 12, 2021·Phytotherapy Research : PTR·Marjan TalebiSaeed Samarghandian
Dec 4, 2020·International Neurourology Journal·Su Jin Kim
May 4, 2021·Frontiers in Pharmacology·Jianwu WangWeibing Li
Jul 3, 2021·International Journal of Molecular Sciences·Yi-Hsuan WuYung-Shun Juan

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Methods Mentioned

BETA
nuclear translocation
Assay
MDA
protein assay

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