Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study

Urology
Ju-Ton HsiehKuo-How Huang

Abstract

To investigate the protective effect of epigallocatechin gallate (EGCG), a green tea extract, on partial bladder outlet obstruction (pBOO)-induced bladder injury in a rat model. The female Sprague-Dawley rats underwent sham or BOO procedures, and were divided into several groups (sham with saline injection, sham with EGCG treatment, BOO with saline injection, and BOO with EGCG treatment). The rats in each group were randomized into 2 groups (48 hours and 30 days after the BOO procedure) for when their bladders were harvested. EGCG (4.5 mg/kg/day) and saline were administered via intraperitoneal injection after the BOO procedure during the study period. Bladder tissue was examined for inflammation, endoplasmic reticulum (ER) stress-related apoptotic markers by Western blot, and histological staining. BOO induced acute bladder injury (hemorrhage, edema, and neutrophil infiltration) after 48 hours. In addition, cystometry showed a decrease in micturition pressure and intercontractile interval. We also observed increased expressions of cyclooxygenase-2, poly(ADP-ribose) polymerase at 48 hours, as well as ER stress markers such as caspase-12 and CCAAT/-enhancer-binding protein homologous protein (CHOP). Treatment with EGCG significa...Continue Reading

References

Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Brahim ChaqourRobert Levin
Nov 20, 2002·The Journal of Clinical Investigation·David Ron
Jun 7, 2005·Biochimica Et Biophysica Acta·Heather L MontieDonald J Degracia
Jan 7, 2006·Cell Death and Differentiation·M Boyce, J Yuan
Jun 24, 2006·Circulation Research·Donna J ThueraufChristopher C Glembotski
Aug 1, 2006·Phytochemistry·Dale G NagleYu-Dong Zhou
Aug 12, 2006·The Journal of Biological Chemistry·Tomohiro YorimitsuDaniel J Klionsky
Nov 2, 2011·Menopause : the Journal of the North American Menopause Society·Yung-Shun JuanChun-Hsiung Huang

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