Epigallocatechin gallate induces GLUT4 translocation in skeletal muscle through both PI3K- and AMPK-dependent pathways

Food & Function
Manabu Ueda-WakagiHitoshi Ashida

Abstract

Our previous report demonstrated that epigallocatechin gallate (EGCg) promotes translocation of glucose transporter 4 (GLUT4) in skeletal muscle. In this study, we investigated the molecular mechanism of GLUT4 translocation by EGCg at the physiological concentration range. In L6 cells, EGCg induced phosphorylation of phosphatidylinositide 3'-kinase (PI3K) and downstream protein kinase C (PKC) λ/ξ without affecting the phosphorylation of insulin receptor and Akt. EGCg-induced GLUT4 translocation was suppressed by RNA interference-mediated knockdown of PI3K and treatment with PKC inhibitor Go6983. Moreover, EGCg increased Rac1 activity and actin remodelling as downstream events of PKCλ/ξ. These results indicate that EGCg induced GLUT4 translocation through a PI3K-dependent pathway, but its mode of action differed from that of insulin. EGCg also induced GLUT4 translocation through a 5'-adenosine monophosphate-activated protein kinase (AMPK)-dependent pathway. 67 kDa laminin receptor, which is a target molecule of EGCg, was not involved in EGCg-induced glucose uptake in L6 cells. The oral administration of EGCg suppressed postprandial hyperglycaemia accompanied by GLUT4 translocation through both PI3K- and AMPK-dependent pathways, ...Continue Reading

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Citations

May 19, 2019·International Journal of Molecular Sciences·Manabu Ueda-WakagiAnd Hitoshi Ashida
Aug 18, 2020·Journal of Clinical Biochemistry and Nutrition·Ken-Yu HironaoYoko Yamashita
Dec 2, 2020·International Journal of Molecular Sciences·Jasminka GiacomettiSnježana Bajek
Dec 17, 2020·Frontiers in Cell and Developmental Biology·Jingxuan LiChangchun Li
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomokazu OhishiMamoru Isemura
Apr 21, 2021·Phytotherapy Research : PTR·Hong ZhangCheng Guo
Jun 10, 2021·Journal of Natural Products·Yoshinori FujimuraHirofumi Tachibana
Jan 18, 2019·Journal of Agricultural and Food Chemistry·Lujuan XingYoshinori Mine
Feb 28, 2020·Journal of Agricultural and Food Chemistry·Liske M Kotzé-Hörstmann, Hanél Sadie-Van Gijsen
Nov 17, 2020·Journal of Agricultural and Food Chemistry·Tomoya KitakazeHitoshi Ashida
Nov 11, 2021·Journal of Agricultural and Food Chemistry·Yujie SunLi Wang

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