Epigenetic age-predictor for mice based on three CpG sites

ELife
Yang HanWolfgang Wagner

Abstract

Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise estimation of chronological age in murine blood samples, too. DBA/2 mice revealed accelerated epigenetic aging as compared to C57BL6 mice, which is in line with their shorter life-expectancy. The three-CpG-predictor provides a simple and cost-effective biomarker to determine biological age in large intervention studies with mice.

References

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Citations

Nov 15, 2018·ELife·Margarita V MeerVadim N Gladyshev
Nov 2, 2019·Molecular Ecology Resources·Dafni Anastasiadi, Francesc Piferrer
Nov 17, 2019·Cells·Christian SchmeerOtto W Witte
Jun 26, 2020·BMC Biology·Yang HanWolfgang Wagner
Mar 27, 2020·Frontiers in Genetics·Yasmeen SalamehNady El Hajj
Apr 8, 2020·International Journal of Molecular Sciences·Quratul AinAlexandra Kretz
Jan 2, 2021·Scientific Reports·Yang HanWolfgang Wagner
Jun 27, 2021·Science Advances·Csaba KerepesiVadim N Gladyshev

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Methods Mentioned

BETA
Chip
RRBS
WGBS
PCR

Software Mentioned

glmnet
R package glmnet
PyroMark Q CpG

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