Abstract
Locally advanced invasive cervical cancer [International Federation of Gynecology and Obstetrics (FIGO) IIB/III] is treated by chemoradiation. The response to treatment is variable within a given FIGO stage. Therefore, the aim of the present study was to evaluate the gene promoter methylation profile and corresponding transcript expression of a panel of six genes to identify genes which could predict the response of patients treated by chemoradiation. In total, 100 patients with invasive cervical cancer in FIGO stage IIB/III who underwent chemoradiation treatment were evaluated. Ten patients developed systemic metastases during therapy and were excluded. On the basis of patient follow-up, 69 patients were chemoradiation-sensitive, whereas 21 were chemoradiation-resistant. Gene promoter methylation and gene expression was determined by TaqMan assay and quantitative real-time PCR, respectively, in tissue samples. The methylation frequency of ESR1, BRCA1, RASSF1A, MLH1, MYOD1 and hTERT genes ranged from 40 to 70%. Univariate and hierarchical cluster analysis revealed that gene promoter methylation of MYOD1, ESR1 and hTERT could predict for chemoradiation response. A pattern of unmethylated MYOD1, unmethylated ESR1 and methylated h...Continue Reading
References
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Mar 27, 2001·International Journal of Cancer. Journal International Du Cancer·G B WismanA G van der Zee
Mar 29, 2001·The Journal of Molecular Diagnostics : JMD·S GattenloehnerA Marx
Apr 4, 2003·International Journal of Cancer. Journal International Du Cancer·Mei Y YuKai F To
Feb 5, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andreas WidschwendterMartin Widschwendter
May 6, 2004·Cancer Research·Gopeshwar NarayanVundavalli V V S Murty
May 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andreas WidschwendterMartin Widschwendter
Jul 15, 2004·Annals of the New York Academy of Sciences·Hannes M MüllerMartin Widschwendter
Nov 9, 2004·Mutation Research·Andrew R Snyder, William F Morgan
Nov 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Partha M Das, Rakesh Singal
Dec 14, 2004·Gynecologic Oncology·Sokbom KangHyo Pyo Lee
Jul 1, 2006·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Amit Kumar AdhyaFiruza D Patel
Dec 7, 2007·Nature Reviews. Genetics·Steven Henikoff
Dec 5, 2008·Gynecologic Oncology·Nicolas WentzensenSophia S Wang
Apr 9, 2009·Acta Obstetricia Et Gynecologica Scandinavica·Jong-Min LeeKyu-Chan Lee
Oct 21, 2009·Molecular Biotechnology·Jörg Tost
Jun 29, 2010·The American Journal of Pathology·Yali ZhaiKathleen R Cho
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Oct 4, 2012·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Ericka WiebeGillian Thomas
Oct 25, 2013·International Journal of Molecular Sciences·Sibaji SarkarMcKenna Longacre
Nov 29, 2013·Cancer Cell International·Bin ZhangJunye Liu
Jan 3, 2015·Nature Reviews. Genetics·Daniel Holoch, Danesh Moazed
Citations
Jul 3, 2007·Gynecologic Oncology·James E KendrickDonald J Buchsbaum
Sep 8, 2004·Gynecologic Oncology·Virach WootipoomParamee Thongsuksai
Jan 31, 2017·EBioMedicine·Lingyu ZhaoChen Huang
Oct 28, 2019·Medicine·Haixia XuXiaojing Kang
Jun 15, 2020·Clinical and Translational Medicine·Chao HuHongping Xia
Mar 6, 2017·Oncology Reports·Zhuolian YinJuxiang Sun
Jun 28, 2016·Oncology Letters·Ming-Yan LiuXiao-Nan Zhao
Apr 30, 2017·Oncology Letters·Zhanzhan XuMin Zhao
Jun 1, 2018·International Journal of Genomics·Chunyang FengTianmin Xu
Aug 14, 2018·Journal of Dairy Science·Xiliang DuXinwei Li
May 19, 2019·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Marie PlanteFrederic Amant
Apr 14, 2017·BMC Cancer·Marjolaine NgolloDominique Bernard-Gallon
Nov 24, 2020·Frontiers in Microbiology·Zhi-Jian ZhuJun Luo
Dec 30, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sophia MastorakiEvi Lianidou
Jan 25, 2021·Pathology, Research and Practice·Sara RavaioliSara Bravaccini
Jul 30, 2021·Clinical and Translational Medicine·Yingxin HanJian Huang