Epigenetic alterations in RASSF1A in human aberrant crypt foci

Carcinogenesis
Emily J GreenspanDaniel W Rosenberg

Abstract

CpG island methylation (CIM) is an epigenetic mechanism for transcriptional silencing that occurs at various stages of colon tumorigenesis. CIM has been found in serrated adenomas and hyperplastic polyps. There is also evidence for hypermethylation in aberrant crypt foci (ACF) that are found in resected colons from cancer patients. Our study addresses promoter methylation of a tumor suppressor gene, RASSF1A, within the colonic epithelium of subjects undergoing screening colonoscopies in the absence of synchronous tumors. Patients included in this study were at elevated risk for colorectal cancer (CRC) based on family history, but without a previously occurring or synchronous colon carcinoma. ACF were identified using close-focus magnifying chromendoscopy and collected by biopsy in situ. We isolated ACF and adjacent normal colonic epithelium by laser capture microdissection (LCM) and studied methylation of the RASSF1A promoter region in ACF and in adjacent normal mucosa. Expression of RASSF1A was verified using quantitative real-time polymerase chain reaction (QRT-PCR). We found that 8.6% (3 out of 35) of ACF had K-ras mutations and 24% (6 out of 25) had RASSF1A hypermethylation. Our results demonstrate that RASSF1A hypermethyla...Continue Reading

References

Sep 1, 1988·The New England Journal of Medicine·B VogelsteinJ L Bos
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Apr 1, 1997·Histopathology·C Di GregorioL Roncucci
Oct 29, 1998·The New England Journal of Medicine·T TakayamaY Niitsu
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Sep 11, 2001·The American Journal of Pathology·A RashidS R Hamilton
May 10, 2002·The American Journal of Pathology·Annie On-On ChanAsif Rashid
May 25, 2002·Molecular and Cellular Biology·Latha ShivakumarMichael A White
May 29, 2002·Oncogene·Manon van EngelandJames G Herman
Aug 29, 2002·Gastroenterology·Jeremy R JassBarbara A Leggett
Feb 25, 2003·The American Journal of Pathology·Seun-Ja ParkTsung-Teh Wu
Sep 27, 2003·Neoplasia : an International Journal for Oncology Research·Jie LiMing You
Oct 14, 2004·Oncogene·Naoto SakamotoHiroaki Fujii
Jun 14, 2005·Biochemistry. Biokhimii︠a︡·G P Pfeifer, R Dammann
Sep 22, 2005·Journal of the National Cancer Institute·Lanlan ShenJean-Pierre J Issa

❮ Previous
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Citations

Nov 26, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Justin J-L WongMegan P Hitchins
Sep 27, 2012·Genetic Testing and Molecular Biomarkers·Shee-Ping ChenCheng-Yoong Pang
Dec 31, 2008·Current Opinion in Gastroenterology·Akshay K Gupta, Robert E Schoen
May 17, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·A YoshidaS F M Derchain
Jan 21, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Dimo DietrichGlen Kristiansen
Jan 26, 2010·PloS One·Victoria GonzaloUNKNOWN Gastrointestinal Oncology Group of the Spanish Gastroenterological Association
Jan 4, 2013·Nutrition Reviews·Manoj KumarBirbal Singh
Jun 22, 2013·Pathology, Research and Practice·Joanne HorneNorman Carr
Mar 20, 2012·Cirugía española·Pablo MenéndezJosé Antonio Rodríguez Montes
Jul 14, 2009·The Journal of Surgical Research·Katharyn E TurnerJohn A Sandoval
Sep 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Serguei P GolovanCecil W Forsberg
Jun 3, 2008·Gastrointestinal Endoscopy·Shaheen Rasheed, Basil Rigas
Apr 17, 2007·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Akshay K GuptaRobert E Schoen
Dec 22, 2006·Cancer Letters·Richard G StevensDaniel W Rosenberg
Jul 26, 2008·Molecular Nutrition & Food Research·Isabel VillegasCatalina Alarcón de la Lastra
Nov 29, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Guro E LindRagnhild A Lothe
Sep 5, 2012·International Journal of Cancer. Journal International Du Cancer·Liang PengXinying Wang
Jun 27, 2012·International Journal of Cancer. Journal International Du Cancer·Maria Sofia FernandesRaquel Seruca
May 7, 2009·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Matthew G MutchPaul F Pinsky
Aug 6, 2013·Analytical Biochemistry·Ratnam S SeelanRobert M Greene
Jun 7, 2014·Molecular Cell·Hariharan EaswaranStephen B Baylin
Aug 23, 2008·Gynecologic Oncology·Aasia SaleemuddinShelley Tworoger
Feb 13, 2007·Cancer Cell·Gijs R van den Brink, G Johan Offerhaus
Mar 23, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jayadev RajuRekha Mehta
Apr 20, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mariana F FragosoLuis F Barbisan
Mar 23, 2010·Acta Cirúrgica Brasileira·Idália Maria Brasil BurlamaquiLusmar Veras Rodrigues
Jan 1, 2010·Cancers·Michael J WargovichJay Morris
May 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julien D F LicchesiJames G Herman

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