Epigenetic control of the expression of a primate-specific microRNA cluster in human cancer cells

Epigenetics : Official Journal of the DNA Methylation Society
Kuo-Wang TsaiWen-chang Lin

Abstract

Many of the known microRNAs (miRNAs) are encoded by polycistronic transcripts and are thought to be co-expressed. In this study, we discovered that the expression of a large miRNA cluster (C19MC) on human chromosome 19 is upregulated by the demethylating agent, 5-aza-2'-deoxycytidine (5-Aza-dC), in AGS gastric cancer cells. We found that C19MC was rarely expressed in most cells, but its expression was restored through DNA demethylation. We confirmed that this miRNA cluster was mainly expressed in the placenta, as previously reported. Furthermore, its expression pattern was highly correlated with the methylation state of a distal CpG-rich region located about 17.6 kb upstream of the miRNA cluster. Using combined bisulfite restriction analysis (COBRA) and bisulfite-sequencing techniques, we determined that this CpG-rich region is hypermethylated in the AGS and HeLa cells, but hypomethylated in the placenta tissue. In conclusion, we demonstrated that the expression pattern of the C19MC was activated in human cancer cells through demethylation of a CpG-rich region.

Citations

Sep 20, 2011·The Journal of Biological Chemistry·Gabriel EadesQun Zhou
Feb 3, 2012·Carcinogenesis·Yu-Lun LiaoWen-Chang Lin
Jul 9, 2010·Human Molecular Genetics·Marie Noguer-DanceJérôme Cavaillé
Nov 2, 2011·Developmental Neuroscience·Jennifer Keck-WherleyZygmunt Galdzicki
Mar 2, 2013·BMC Biology·Anthony O Olarerin-GeorgeJohn B Hogenesch
Jul 7, 2012·Journal of Molecular Endocrinology·Valentina VairaSabrina Corbetta
Nov 20, 2013·Digestive Diseases and Sciences·Jiaojiao MaDaiming Fan
Oct 15, 2013·Experimental and Molecular Pathology·James MathewsJohn D Pfeifer
Feb 18, 2011·Gastroenterology·Sara ToffaninJosep M Llovet
Jan 15, 2015·Leukemia & Lymphoma·Alfons NavarroMariano Monzó
Feb 26, 2013·Journal of Reproductive Immunology·Diana M Morales-PrietoUdo R Markert
May 12, 2012·Biochemical and Biophysical Research Communications·Inga FlorJörn Bullerdiek
Mar 23, 2011·Mutation Research·Tanja KunejGeorge Adrian Calin
Mar 1, 2011·Journal of Reproductive Immunology·Diana M Morales Prieto, Udo R Markert
Oct 1, 2011·International Journal of Cancer. Journal International Du Cancer·Kuo-Wang TsaiWen-Chang Lin
Mar 23, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jean-Francois MouilletYoel Sadovsky
Mar 1, 2012·The Journal of Pathology·Inga Flor, Jörn Bullerdiek
Mar 16, 2012·Genes, Chromosomes & Cancer·Kuo-Wang TsaiWen-Chang Lin
Mar 21, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Claudia AugelloSilvano Bosari
Oct 30, 2013·Molecular Reproduction and Development·Benjamin J HaleJason W Ross
Sep 9, 2010·Essays in Biochemistry·Asli Silahtaroglu, Jan Stenvang
Jun 23, 2012·Placenta·D M Morales-PrietoU R Markert
Jul 22, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Matthew A MaccaniCarmen J Marsit
Nov 17, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Michael GirardotRobert Feil
Mar 15, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Julia LiepKlaus Jung
Oct 2, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Sam BuckberryClaire T Roberts
Nov 16, 2010·Developmental Cell·María Berdasco, Manel Esteller
Aug 3, 2011·The American Journal of Pathology·Ming JiTimothy W McKeithan

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