Epigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer

Gene
Moonmoon DebSamir Kumar Patra

Abstract

Caveolin-1 (CAV1) is an important structural component of cellular caveolae involved in cell signaling. CAV1 gene on/off regulatory mechanism in multiple diseases, including cancer is not clearly understood. The tumor suppressor versus oncogene paradox of CAV1 during tumor development tempted us to investigate the role for the epigenetic drift of CAV1 gene regulation. We have analyzed CAV1 gene expression and associated epigenetic marks (DNA methylation and histone 3 modifications) in the CAV1 promoter in two colon cancer cell lines, under treatment with well established epigenetic modulators, AZA, SAM, TSA and SFN at varying concentrations. CAV1 gene promoter DNA methylation and histone modifications were analyzed by DNA methylation specific PCR, bisulphite modification of DNA and ChIP analyses following PCR respectively. Ectopic expression of CAV1 by epigenetic modulators inhibits colon cancer cell growth. CAV1 promoter DNA remains unmethylated before and after treatment with epigenetic modulators, which confirmed that DNA methylation is not the regulator of CAV1 expression in colon cancer. There was enrichment of H3K4me3 and H3K9AcS10P and depletion of H3K9me3 modifications around the CAV1 promoter. Our data provides novel i...Continue Reading

References

Aug 29, 2001·The Journal of Biological Chemistry·N Cervoni, M Szyf
Sep 21, 2001·Biochemical and Biophysical Research Communications·S K PatraR Dahiya
Sep 28, 2002·Nature·Helena Santos-RosaTony Kouzarides
Jul 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario F FragaManel Esteller
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
Jan 2, 2008·Biochimica Et Biophysica Acta·Samir Kumar Patra
Apr 19, 2008·Journal of Cellular and Molecular Medicine·C HaboldR Schneider-Stock
Nov 11, 2011·The Journal of Pathology·Edith Yuk Ting TseJudy Wai Ping Yam
Dec 14, 2011·Cancer Metastasis Reviews·Moonmoon DebSamir Kumar Patra
Jun 19, 2012·Clinical Epigenetics·Aditi PatraSamir Kumar Patra
Jun 30, 2012·PloS One·Huafei ZouFerruccio Galbiati
Aug 7, 2013·Human Molecular Genetics·Andrew E TeschendorffStephan Beck
Mar 29, 2014·Cellular and Molecular Life Sciences : CMLS·Moonmoon DebSamir Kumar Patra
Sep 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Moonmoon DebSamir Kumar Patra

❮ Previous
Next ❯

Citations

Jan 12, 2019·Cancer Investigation·Emaad KhansurRicardo J Komotar
Jul 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Fei QiuJing Wang
May 28, 2020·Cancer Metastasis Reviews·L SimónA F G Quest
Feb 12, 2017·Chonnam Medical Journal·Kim Cuc Thi Nguyen, Kyung A Cho
Jan 1, 2020·Clinical Proteomics·Saira SaleemChris Sutton
Apr 4, 2017·Journal of Cellular Biochemistry·Xiao-Jun XieHai-Wen Zhuang
Nov 14, 2018·Best Practice & Research. Clinical Endocrinology & Metabolism·Monica Muratori, Christian De Geyter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.