Epigenetic formation of lactate dehydrogenase isozymes in the house mouse, Mus musculus

The Journal of Experimental Zoology
K I YamamuraC L Markert

Abstract

The origin of mouse lactate dehydrogenase (LDH) sub-bands was investigated by using our miniaturized polyacrylamide gel electrophoretic apparatus. Mouse LDH isozymes are generated by combinations of three types of A subunit, the primary type and two epigenetically modified forms. These are designated A1, A2, and A3 in the order of their electrophoretic mobilities towards the anode. The A1 subunit arises from the covalent binding of molecules of glutathione through disulfide bonds to the original subunit, A3. The A2 subunit arises from the covalent binding of molecules of cysteine through disulfide bonds to the A3 subunit. All isozymes can be explained as tetramers composed of the three kinds of A subunit (A1, A2, or A3) in combination with B subunits to yield a total of 35 isozymes. The kinetic properties of these sub-bands were also examined. There was no difference between A24 and A34 in the Km for pyruvate and for lactate. Thermostability at 56 degrees C was greater for A34 than for A24. The activities of tetramers at the electrophoretic position of A3B1 and A4 in extracts containing all five isozymes were increased by treatment of the extracts with high concentrations of reduced glutathione or cysteine with the concomitant ...Continue Reading

References

Nov 1, 1979·Analytical Biochemistry·Z I Ogita, C L Markert
Jan 7, 1969·Biochimica Et Biophysica Acta·N P Dudman, B Zerner
May 17, 1973·Biochimica Et Biophysica Acta·N P Dudman, B Zerner
Jan 1, 1974·Archives of Biochemistry and Biophysics·L D Ryan, C S Vestling
Oct 15, 1968·Experientia·C L Markert, G S Whitt
Jun 14, 1968·Annals of the New York Academy of Sciences·C L Markert
Aug 15, 1969·Biochemical and Biophysical Research Communications·N P Dudman
Apr 5, 1963·Science·P J FRITZ, K B JACOBSON
Aug 6, 1963·Biochimica Et Biophysica Acta·L A COSTELLO, N O KAPLAN
Dec 28, 1964·Annals of the New York Academy of Sciences·W H ZINKHAML J CLOWRY
May 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·C L Markert, F Møller

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Citations

Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E Vonwyl
Nov 1, 1979·Analytical Biochemistry·Z I Ogita, C L Markert

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