Epigenetic mechanisms and Sp1 regulate mitochondrial citrate carrier gene expression

Biochemical and Biophysical Research Communications
V IacobazziFerdinando Palmieri

Abstract

This study investigates the transcriptional role of the mitochondrial CIC proximal promoter. The wild-type (but not methylated) -335/-20 bp region of the CIC gene confers gene reporter activity, and the five wild-type (but not methylated) Sp1 binding elements in this region bind human recombinant Sp1. The DNA demethylating agent AzaC or the histone acetylating agent TSA increases CIC transcript and protein levels as well as the binding of Sp1 and of acetylated histones to the -335/-20 bp region of the CIC promoter in SK-N-SH cells but not in HepG2 cells; when untreated these cells exhibit low and high levels of gene expression, respectively. Finally, Sp1 silencing decreases proximal promoter-driven gene reporter activity as well as CIC mRNA and CIC protein in both untreated HepG2 cells and AzaC- and TSA-treated SK-N-SH cells. These results show that methylation, histone acetylation and Sp1 are important in the transcriptional regulation of the CIC proximal promoter.

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Citations

Sep 11, 2012·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·A MengaV Iacobazzi
May 6, 2014·Archives of Toxicology·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Sep 7, 2013·Gene·Vittoria InfantinoVito Iacobazzi
Jul 30, 2011·Biochemical and Biophysical Research Communications·Vittoria InfantinoVito Iacobazzi
Aug 12, 2009·Biochemical and Biophysical Research Communications·Vito IacobazziFerdinando Palmieri
May 19, 2009·Biochemical and Biophysical Research Communications·Vito IacobazziFerdinando Palmieri
Jul 27, 2011·The Biochemical Journal·Vittoria InfantinoVito Iacobazzi
Sep 3, 2014·Mitochondrion·Björn KruspigVladimir Gogvadze
Dec 7, 2010·Biochemical and Biophysical Research Communications·Paolo ConvertiniVito Iacobazzi
May 20, 2015·Chemico-biological Interactions·Paolo ConvertiniVittoria Infantino
Jul 4, 2015·Hormones & Cancer·Ivana JochmanováKarel Pacak
Jul 30, 2014·Biochimica Et Biophysica Acta·Vittoria InfantinoFerdinando Palmieri
Jan 20, 2015·The International Journal of Biochemistry & Cell Biology·Alessio MengaFerdinando Palmieri
Sep 21, 2013·Biochemical and Biophysical Research Communications·Vittoria InfantinoAlessio Menga
Jun 10, 2009·Biochemical and Biophysical Research Communications·Vito IacobazziFerdinando Palmieri
Nov 5, 2016·Immunology·Paolo ConvertiniVittoria Infantino
Oct 12, 2016·Biological Chemistry·Vito IacobazziFerdinando Palmieri
Oct 24, 2019·Reviews in Endocrine & Metabolic Disorders·Andrea PalermoAlberto Falchetti
Jan 22, 2014·Biological Chemistry·Vito Iacobazzi, Vittoria Infantino
Dec 29, 2019·Cancers·Simona TodiscoVittoria Infantino
Nov 19, 2020·Oxidative Medicine and Cellular Longevity·Anna SantarsieroGiuseppe Martelli

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