Abstract
BMP4 is the critical gene of primordial germ cell formation in mammal, however, the mechanism of PGCs formation in chicken still unknown. In this research, we compared the evolution relationship of different species. Although the protein sequence is highly conservative between mouse, human and chicken, promotors vary among avian and mammal species. Therefore, it is easily to predict that there would be different regulation mechanism of Bmp4 expression in chicken. Here, we elucidate the function of chicken Bmp4 during PGCs formation. In vivo, Bmp4 can promote PGCs development and migration, and increase the expression of key genes (Cvh, c-kit, cxcr4, etc.). Whereas, the expression of these genes will decrease after knocking out Bmp4. After over-expression and knockout Bmp4 in vitro, we found that overexpression of Bmp4 could promote the formation of embryoid bodies (EB) and up-regulate the key genes of PGCs formation and migration, while knockout Bmp4 could inhibit the formation of embryoid bodies and decrease the expression of related genes. Flow and indirect immunofluorescence also indicated the same result. These all results proved that chicken Bmp4 could also promote the formation of PGCs. Furthermore, dual-luciferase activi...Continue Reading
References
Feb 27, 1999·Genes & Development·K A LawsonB L Hogan
Nov 5, 2003·Development·Cassandra G Extavour, Michael Akam
Aug 7, 2004·Mechanisms of Development·J N PetitteZ Yang
Aug 7, 2004·Mechanisms of Development·Mary E Delany
Oct 30, 2004·Molecular Reproduction and Development·Daiji OkamuraYasuhisa Matsui
Mar 8, 2005·Development·Stéphane D VincentElizabeth J Robertson
Mar 19, 2005·Genes & Development·Makoto TachibanaYoichi Shinkai
Jun 9, 2006·Nature·Marie-Cecile van de LavoirRobert J Etches
Sep 20, 2007·Poultry Science·Y NakamuraT Tagami
May 22, 2010·Stem Cells and Development·Marc HillerCandace L Kerr
Feb 22, 2012·Histochemistry and Cell Biology·Jeong-Oh ShinHan-Sung Jung
Apr 27, 2012·Gut·Tatiana IvanovaPatrick Tan
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joni MacdonaldMichael J McGrew
Nov 6, 2012·Cold Spring Harbor Perspectives in Biology·Mitinori Saitou, Masashi Yamaji
Dec 18, 2013·Developmental Cell·Shinya AramakiMitinori Saitou
Dec 18, 2013·Biology of Reproduction·Yonghong SongMarie-Cecile van de Lavoir
Jan 31, 2014·International Journal of Cancer. Journal International Du Cancer·Wen-Tsen FangShih Sheng Jiang
Apr 1, 2015·Cardiovascular Research·Jian WangWenju Lu
Aug 19, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kimberly P KeilChad M Vezina
Aug 8, 2018·Cell Death & Disease·Jieying PengLu Gao
Oct 5, 2018·Nature·Jingchao ZhangIan Chambers
Jan 19, 2019·Journal of Cellular Biochemistry·Jing JinBichun Li
Feb 6, 2019·Cell Death & Disease·Bo TangSongqing He
Sep 15, 2019·Morphologie : Bulletin De L'Association Des Anatomistes·M LejongS Louryan
Aug 5, 2020·Journal of Cellular Physiology·Chen ZhangBichun Li
Aug 28, 2020·Frontiers in Genetics·Kai JinBichun Li