Epigenetic Mutation of RAV6 Affects Leaf Angle and Seed Size in Rice

Plant Physiology
Xiangqian ZhangXianwei Song

Abstract

Heritable epigenetic variants of genes, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Epialleles may also provide a new source of beneficial traits for crop breeding, but very few epialleles related to agricultural traits have been identified in crops. Here, we identified Epi-rav6, a gain-of-function epiallele of rice (Oryza sativa) RELATED TO ABSCISIC ACID INSENSITIVE3 (ABI3)/VIVIPAROUS1 (VP1) 6 (RAV6), which encodes a B3 DNA-binding domain-containing protein. The Epi-rav6 plants show larger lamina inclination and smaller grain size; these agronomically important phenotypes are inherited in a semidominant manner. We did not find nucleotide sequence variation of RAV6. Instead, we found hypomethylation in the promoter region of RAV6, which caused ectopic expression of RAV6 in Epi-rav6 plants. Bisulfite analysis revealed that cytosine methylation of four CG and two CNG loci within a continuous 96-bp region plays essential roles in regulating RAV6 expression; this region contains a conserved miniature inverted repeat transposable element transposon insertion in cultivated rice genomes. Overexpression of RAV6 in the wild type phenocopied the Epi-rav6 phenotype. The brassinosteroid (BR) receptor BR I...Continue Reading

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Citations

Aug 22, 2017·Frontiers in Plant Science·Rakesh KumarAnirudh Kumar
Nov 3, 2020·Frontiers in Plant Science·Zhuoyun Wei, Jia Li
Feb 7, 2021·Scientific Reports·Biruk A FeyissaAbdelali Hannoufa
May 23, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhixuan DuHaohua He
Dec 18, 2016·The Plant Journal : for Cell and Molecular Biology·Matthew GillihamStuart J Roy
Jul 30, 2021·Frontiers in Plant Science·Ke ChenMariusz Jaremko
Aug 8, 2021·International Journal of Molecular Sciences·Seonghoe JangYue-Ie Hsing
Aug 28, 2021·Biology·Ioanna KakoulidouStéphane Maury
Jul 25, 2021·Journal of Experimental Botany·Dharmendra SinghMadan Pal

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