Epigenetic programming via histone methylation at WRKY53 controls leaf senescence in Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
Nicole AyKlaus Humbeck

Abstract

Leaf senescence, the final step of leaf development, involves extensive reprogramming of gene expression. Here, we show that these processes include discrete changes of epigenetic indexing, as well as global alterations in chromatin organization. During leaf senescence, the interphase nuclei show a decondensation of chromocenter heterochromatin, and changes in the nuclear distribution of the H3K4me2, H3K4me3, and the H3K27me2 and H3K27me3 histone modification marks that index active and inactive chromatin, respectively. Locus-specific epigenetic indexing was studied at the WRKY53 key regulator of leaf senescence. During senescence, when the locus becomes activated, H3K4me2 and H3K4me3 are significantly increased at the 5' end and at coding regions. Impairment of these processes is observed in plants overexpressing the SUVH2 histone methyltransferase, which causes ectopic heterochromatization. In these plants the transcriptional initiation of WRKY53 and of the senescence-associated genes SIRK, SAG101, ANAC083, SAG12 and SAG24 is inhibited, resulting in a delay of leaf senescence. In SUVH2 overexpression plants, significant levels of H3K27me2 and H3K27me3 are detected at the 5'-end region of WRKY53, resulting in its transcription...Continue Reading

References

Jul 24, 1992·Cell·H C LinR G Anderson
Dec 1, 1992·The Journal of Cell Biology·E SmytheS L Schmid
Sep 1, 1991·The Journal of Cell Biology·S L Schmid, E Smythe
Sep 1, 1991·The Journal of Cell Biology·H C LinR G Anderson
Jan 1, 1990·Methods in Enzymology·J R Bartles, A L Hubbard
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D J ChinF M Brodsky
Jan 1, 1983·The Journal of Cell Biology·A L HubbardA Ma
Sep 1, 1995·The Journal of Cell Biology·C DesnosR B Kelly
Jan 1, 1993·The Journal of Cell Biology·L L CarterS L Schmid
Jan 5, 1996·The Journal of Biological Chemistry·H S ShpetnerR B Vallee
Jun 1, 1996·The Journal of Cell Biology·K TakeiP De Camilli
Nov 1, 1996·The Journal of Cell Biology·T RoweW E Balch
Jan 1, 1997·Annual Review of Biochemistry·S L Schmid
Sep 23, 1997·The Journal of Cell Biology·M A WestM S Robinson
Jan 10, 1998·The EMBO Journal·A B MuhlbergS L Schmid
Feb 7, 1998·Science·A Hall
Apr 29, 1999·Cell·S SpringerR Schekman
Mar 15, 2001·Current Opinion in Cell Biology·W E Miller, R J Lefkowitz

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Citations

Nov 3, 2010·Plant Physiology·Alessandro BottonAngelo Ramina
Nov 7, 2012·BMC Plant Biology·Clelia De-la-PeñaManuel L Robert-Díaz
Jun 3, 2006·Developmental Cell·Eric MaciaTomas Kirchhausen
Apr 17, 2015·TheScientificWorldJournal·Aditya Banerjee, Aryadeep Roychoudhury
Jul 5, 2016·Frontiers in Plant Science·Ujjal J PhukanRakesh K Shukla
Nov 2, 2014·Molecular Biology of the Cell·Sandra L Schmid
Jul 21, 2004·The Journal of Cell Biology·Genkichi IzumiYoshimi Takai
May 24, 2008·The Biochemical Journal·Joshua Z Rappoport
Jan 14, 2017·Physical Review. E·Gaurav Kumar, Anirban Sain
Jul 21, 2004·The Journal of Biological Chemistry·Byeong Doo SongSandra L Schmid
Feb 9, 2007·Journal of Cell Science·Karen Newell-LitwaVictor Faundez

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