Epigenetic Regulation in Early Childhood: A Miniaturized and Validated Method to Assess Histone Acetylation

International Archives of Allergy and Immunology
Hani HarbHarald Renz

Abstract

Chronic inflammatory diseases including allergies and asthma are the result of complex interactions between genes and environmental factors. Epigenetic mechanisms comprise a set of biochemical reactions that regulate gene expression. In order to understand the cause-effect relationship between environmental exposures and disease development, methods capable of assessing epigenetic regulation (also) in large cohorts are needed. For this purpose, we developed and evaluated a miniaturized chromatin immunoprecipitation (ChIP) assay allowing for a cost-effective assessment of histone acetylation of candidate genes in a quantitative fashion. This method was then applied to assess H3 and H4 histone acetylation changes in cord blood (CB) samples from an established cohort of Australian children exposed in the fetal period to either very low or very high levels of maternal folate. Our ChIP assay was validated for a minimum requirement of 1 × 105 target cells (e.g. CD4+ T cells). Very high levels of maternal folate were significantly associated with increased H3/H4 acetylation at GATA3 and/or IL9 promoter regions in CD4+ T cells in CB. We developed a ChIP method allowing reliable assessment of H3/H4 acetylation using 1 × 105 cells only. ...Continue Reading

Citations

Mar 9, 2017·European Journal of Immunology·Kathrin KlarClarissa Prazeres da Costa
Jun 28, 2017·International Archives of Allergy and Immunology·Evelyn Xiu Ling LooLynette Pei-Chi Shek
Nov 3, 2016·Current Opinion in Pediatrics·Hani HarbDaniel P Potaczek
Jan 13, 2018·PloS One·Kenneth R EyringDavid A Schwartz
Mar 3, 2019·International Journal of Molecular Sciences·Nathalie AcevedoDaniel P Potaczek
Jul 2, 2019·Journal of Leukocyte Biology·Adam J FikeAlison J Carey
Sep 13, 2017·Frontiers in Immunology·Meike KespohlAlexander Visekruna
May 26, 2018·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Bilal Alaskhar AlhamweDaniel P Potaczek
May 22, 2019·BioMed Research International·Hani HarbAnnika Scheynius
Mar 23, 2017·Epigenomics·Daniel P PotaczekJörg Tost
Oct 20, 2020·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Fei HuangYa-Nan Li
Oct 23, 2020·Nutrients·Bilal Alashkar AlhamweMachteld M Tiemessen
Mar 7, 2021·Nutrients·Nathalie AcevedoBetty C A M van Esch
Mar 9, 2021·The Journal of Allergy and Clinical Immunology·Matthew LacorciaClarissa Prazeres da Costa
Jul 10, 2021·Pediatric Research·Jennifer Bermick, Matthew Schaller
Aug 30, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Johanna TheodorouBianca Schaub

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