Epigenetic regulation of bovine spermatogenic cell-specific gene boule

PloS One
Wang YaoQ Li

Abstract

Non-primate mammals have two deleted azoospermia (DAZ) family genes, DAZL and Boule; genes in this family encode RNA-binding proteins essential for male fertility in diverse animals. Testicular DAZL transcription is regulated by epigenetic factors such as DNA methylation. However, nothing is known about the epigenetic regulation of Boule. Here, we explored the role of DNA methylation in the regulation of the bovine Boule (bBoule) gene. We found that a long CpG island (CGI) in the bBoule promoter was hypermethylated in the testes of cattle-yak hybrids with low bBoule expression, whereas cattle had relatively low methylation levels (P < 0.01), and there was no difference in the methylation level in the short CGI of the gene body between cattle and cattle-yak hybrids (P > 0.05). We identified a 107 bp proximal core promoter region of bBoule. Intriguingly, the differences in the methylation level between cattle and cattle-yak hybrids were larger in the core promoter than outside the core promoter. An in vitro methylation assay showed that the core promoter activity of bBoule decreased significantly after M.SssI methylase treatment (P < 0.01). We also observed dramatically increased bBoule transcription in bovine mammary epithelial ...Continue Reading

References

Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·E Y XuR A Pera
Dec 25, 2002·Human Molecular Genetics·Eugene Yujun XuRenee A Reijo Pera
May 22, 2003·Oncology·Kiyoshi AsadaToshikazu Ushijima
Aug 30, 2003·Science·Masayuki TsudaYumiko Saga
Apr 9, 2004·The Journal of Clinical Endocrinology and Metabolism·C Marc LuetjensJörg Gromoll
May 14, 2004·International Journal of Andrology·Pauline H Yen
Jan 20, 2006·Genome Biology·Dawid EckertHubert Schorle
Jan 21, 2006·Development Genes and Evolution·Joyce Y TungJörg Gromoll
Nov 7, 2008·Molecular Genetics and Genomics : MGG·Qingbo ZhangZ Xie
Mar 14, 2009·Differentiation; Research in Biological Diversity·Katja LinherJulang Li
Nov 3, 2009·Fertility and Sterility·Saher Sue HammoudDouglas T Carrell
Jan 7, 2010·Nature Reviews. Genetics·Mary Ann Handel, John C Schimenti
Feb 23, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Wei WuXinru Wang
Mar 10, 2010·Molecular and Cellular Endocrinology·Kevin C KnowerColin D Clyne
Mar 26, 2010·Human Molecular Genetics·Michael J W Vangompel, Eugene Yujun Xu
Jul 9, 2010·Nature·Alika K MaunakeaJoseph F Costello
Aug 6, 2010·Human Reproduction·Paulo Navarro-CostaCarlos E Plancha
Mar 23, 2011·Human Molecular Genetics·Vanessa T Angeles Julaton, Renee A Reijo Pera
Jun 2, 2011·Genome Research·Aimée M DeatonAdrian Bird
Oct 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Huw T JenkinsThomas A Edwards
Feb 24, 2012·Genome Research·Sylvain GuibertMichael Weber
Jun 14, 2012·Journal of Assisted Reproduction and Genetics·Ruth KläverJörg Gromoll
Aug 14, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Cristina CamprubíJoan Blanco
Oct 12, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jennifer F HughesDavid C Page
Nov 8, 2012·PLoS Genetics·Karen M BerkowitzKlaus H Kaestner
Feb 21, 2013·Reproduction, Fertility, and Development·Byunghyuk KimJae-Seung Paick
May 7, 2013·Molecular Biology Reports·Mingzhao LiJinlian Hua
Aug 27, 2013·Epigenetics : Official Journal of the DNA Methylation Society·John C RotondoFernanda Martini
Jan 29, 2014·Reproduction in Domestic Animals = Zuchthygiene·V Kasimanickam, R Kasimanickam
Mar 13, 2014·Nucleic Acids Research·Alice Gutjahr, Shuang-yong Xu
Jul 30, 2014·Nature Reviews. Genetics·Bryony Jones
Aug 1, 2014·Nature·Hongshan GuoJie Qiao
Aug 1, 2014·Nature·Zachary D SmithAlexander Meissner
Aug 5, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Stephany CorrêaEliana Abdelhay
Oct 21, 2014·PLoS Genetics·Stefano ComazzettoDónal O'Carroll

Citations

Apr 1, 2018·Reproduction in Domestic Animals = Zuchthygiene·N SarovaR Arora
Sep 19, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xiaoli LiuHongyan Xu
Sep 24, 2016·Reproduction in Domestic Animals = Zuchthygiene·B LiQ Li

Related Concepts

Cattle
Epithelial Cells
Fertility
Gene Deletion
Genes
Infertility
Methylation
Transcription, Genetic
RNA-Binding Proteins
Promoter

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.