Epigenetic regulation of kappa opioid receptor gene in neuronal differentiation.

Neuroscience
Sung Wook ParkL-N Wei

Abstract

The gene of mouse kappa opioid receptor (KOR) utilizes two promoters, P1 and P2. P1 is active in various brain areas and constitutively in P19 mouse embryonal carcinoma cells. P2 is active in limited brain stem areas of adult animals and only in late differentiated cells of P19 induced for neuronal differentiation in the presence of nerve growth factor (NGF). NGF response of P2 was found to be mediated by a specific binding site for transcription factor activation protein 2 (AP2) located in P2. Electrophoretic gel shift assay showed specific binding of this AP2 site by AP2beta, but not AP2alpha. Knockdown of endogenous AP2beta with siRNA abolished the stimulating effect of NGF on the expression of transcripts driven by P2. Binding of endogenous AP2beta on the endogenous KOR P2 chromatin region was also confirmed by chromatin immunoprecipitation. The effect of NGF was inhibited by LY2942002 (phosphatidylinositol 3-kinase, PI3K inhibitor), suggesting that PI3K was involved in signaling pathway mediating the effect of NGF stimulation on KOR P2. The chromatin of P2 in P19 was found to be specifically modified following NGF stimulation, which included demethylation at Lys9 and dimethylation at Lys4 of histone H3 and was consistent w...Continue Reading

References

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Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jing BiLi-Na Wei

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Citations

Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nien-Pei TsaiLi-Na Wei
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shlomit Flaisher-GrinbergLi-Na Wei
Sep 28, 2010·Annual Review of Pharmacology and Toxicology·Li-Na Wei, Horace H Loh
May 15, 2015·Journal of Cellular Biochemistry·Iraia MuñoaNerea Subirán
Jun 20, 2012·Trends in Neurosciences·Eduardo R ButelmanMary Jeanne Kreek
Oct 2, 2009·Peptides·Richard J Bodnar
Jun 3, 2009·Stem Cells·Anna GolebiewskaLyle Armstrong
Jun 15, 2012·Proteomics·Andrea M BrunnerIsabelle M Mansuy
Apr 6, 2016·The International Journal of Biochemistry & Cell Biology·Shalini DograPrem N Yadav
Apr 30, 2011·Physiological Reviews·Johannes GräffLi-Huei Tsai
Jan 27, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Olga KononenkoGeorgy Bakalkin

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