Epigenetic regulation of polyomavirus JC involves acetylation of specific lysine residues in NF-κB p65

Journal of Neurovirology
Hassen S WolleboMartyn K White

Abstract

Progressive multifocal leukoencephalopathy (PML) is a fatal demyelinating disease caused by neurotropic polyomavirus, JC virus (JCV), a virus that causes lytic infection of CNS glial cells. After primary infection, JCV is controlled by the immune system but virus persists asymptomatically. Rarely, when immune function is impaired, it can reemerge to cause PML. The mechanisms of JCV persistence and reactivation are not well understood but our earlier work implicated epigenetic control by protein acetylation since histone deacetylase inhibitors such as trichostatin A (TSA) strongly stimulate JCV transcription. Since both TNF-α and TSA activate JCV transcription via the same unique NF-κB site in the JCV control region, we investigated a role for acetylation of NF-κB in JCV regulation. A site-directed mutagenesis strategy was employed targeting the known lysine acetylation sites of NF-κB p65: K218, K221, and K310. We individually mutated each lysine to arginine, which cannot be acetylated and retains a positive charge like lysine. K218R and K221R impaired transactivation of JCV early promoter transcription either alone or combined with TSA treatment or coexpression of acetyltransferase transcriptional coactivator p300 but K310R was...Continue Reading

References

Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E GerritsenT Collins
Nov 29, 2002·The EMBO Journal·Lin-feng ChenWarner C Greene
Aug 18, 2004·Biochemical Pharmacology·Vincent Quivy, Carine Van Lint
Oct 1, 2004·Chembiochem : a European Journal of Chemical Biology·M Lienhard SchmitzMichael Kracht
Apr 21, 2006·Immunological Reviews·Alexander Hoffmann, David Baltimore
Jul 1, 2008·Trends in Biochemical Sciences·Miriam CalaoCarine Van Lint
Jul 31, 2009·Journal of Virology·Martyn K WhiteKamel Khalili
Sep 15, 2009·Virus Research·Luca RomagnoliMartyn K White
Oct 12, 2010·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Joseph R Berger
Dec 28, 2010·Journal of Neuroimmunology·Hassen S WolleboMartyn K White
Jan 14, 2011·The Journal of Infectious Diseases·Martyn K White, Kamel Khalili
Sep 22, 2011·Reviews in Medical Virology·Eleonora TavazziKamel Khalili
Dec 14, 2011·Cleveland Clinic Journal of Medicine·Joseph R Berger
Mar 23, 2013·Trends in Microbiology·Kotaro ShirakawaEric Verdin
Aug 27, 2013·Virology Journal·Hassen S WolleboMartyn K White
Oct 14, 2014·PloS One·Martyn K WhiteHassen S Wollebo

❮ Previous
Next ❯

Citations

Aug 27, 2015·Reviews in Medical Virology·Etienne Xavier KellerMaurizio Provenzano
Jun 10, 2009·Seminars in Cancer Biology·Melissa S Maginnis, Walter J Atwood
Mar 24, 2016·Journal of Neurovirology·Hassen S WolleboMartyn K White
Jun 24, 2017·Annual Review of Virology·Sheila A Haley, Walter J Atwood
Aug 9, 2016·Annual Review of Virology·Michael J Imperiale, Mengxi Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.