Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3.

The Journal of Biological Chemistry
Hiromu TanakaKazuhiko Igarashi

Abstract

B lymphocyte-induced maturation protein 1 (Blimp-1) encoded by Prdm1 is a master regulator of plasma cell differentiation. The transcription factor Bach2 represses Blimp-1 expression in B cells to stall terminal differentiation, by which it supports reactions such as class switch recombination of the antibody genes. We found that histones H3 and H4 around the Prdm1 intron 5 Maf recognition element were acetylated at higher levels in X63/0 plasma cells expressing Blimp-1 than in BAL17 mature B cells lacking its expression. Conversely, methylation of H3-K9 was lower in X63/0 cells than BAL17 cells. Purification of the Bach2 complex in BAL17 cells revealed its interaction with histone deacetylase 3 (HDAC3), nuclear co-repressors NCoR1 and NCoR2, transducin β-like 1X-linked (Tbl1x), and RAP1-interacting factor homolog (Rif1). Chromatin immunoprecipitation confirmed the binding of HDAC3 and Rif1 to the Prdm1 locus. Reduction of HDAC3 or NCoR1 expression by RNA interference in B cells resulted in an increased Prdm1 mRNA expression. Bach2 is suggested to cooperate with HDAC3-containing co-repressor complexes in B cells to regulate the stage-specific expression of Prdm1 by writing epigenetic modifications at the Prdm1 locus.

References

Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·P DhordainD Leprince
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SawadoM Groudine
Mar 16, 2002·Nature Immunology·Tien-An Yang ShihMichel C Nussenzweig
Dec 18, 2002·The EMBO Journal·Akihisa MatsuyamaMinoru Yoshida
Dec 28, 2002·Genes & Development·Matthew G GuentherMitchell A Lazar
Jan 10, 2004·Methods in Enzymology·Yoshihiro Nakatani, Vasily Ogryzko
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jiying SunKazuhiko Igarashi
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roger Sciammas, Mark M Davis
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chainarong TunyaplinKathryn L Calame
Oct 20, 2004·The Journal of Experimental Medicine·Axel KalliesStephen L Nutt
Oct 19, 2006·The Journal of Biological Chemistry·Kyoko OchiaiKazuhiko Igarashi
Feb 8, 2008·International Immunology·Kyoko OchiaiKazuhiko Igarashi
Jun 17, 2008·Current Opinion in Immunology·Kathryn Calame
Feb 20, 2010·The Journal of Biological Chemistry·Akihisa OsakabeHitoshi Kurumizaka
Mar 2, 2011·Cell Research·Diana C Hargreaves, Gerald R Crabtree
Aug 2, 2012·The EMBO Journal·Satoshi YamazakiHisao Masai
Jan 12, 2013·Science·Michal ZimmermannTitia de Lange
Jan 12, 2013·Science·Michela Di VirgilioMichel C Nussenzweig
Jun 4, 2013·Nature·Rahul RoychoudhuriNicholas P Restifo
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shin-ichi TsukumoTakashi Saito
Oct 16, 2013·The Journal of Experimental Medicine·Atsushi NakamuraKazuhiko Igarashi
Dec 25, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eui Ho KimM Suresh
Apr 1, 2014·The Tohoku Journal of Experimental Medicine·Kazuhiko Igarashi, Miki Watanabe-Matsui
Apr 4, 2014·Nature Communications·Makoto KuwaharaMasakatsu Yamashita
Aug 16, 2014·Immunological Reviews·Kazuhiko IgarashiAkihiko Muto
Feb 18, 2015·Nature·Golnaz VahediJohn J O'Shea

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Citations

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Oct 28, 2016·The Journal of Pathology·Werna Tc Uniken VenemaEleonora Am Festen
Mar 16, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuichi MiuraAkihiko Muto
Jun 22, 2016·Nature Immunology·Tom Sidwell, Axel Kallies
Jul 14, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natalie A RobertsValerie Horsley
Jun 27, 2018·Molecular and Cellular Biology·Hiroki SekineHozumi Motohashi
Aug 8, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea Di Pietro, Kim L Good-Jacobson
Oct 6, 2019·Nucleic Acids Research·Kristy R StengelScott W Hiebert
Sep 27, 2018·Nature Immunology·Hiroki KatoKazuhiko Igarashi
Apr 4, 2019·Frontiers in Immunology·Nely Rodriguez-ZhurbenkoAna M Hernandez
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Oct 6, 2020·Frontiers in Cell and Developmental Biology·I-Chung ChenJing-Ping Liou
Jan 9, 2020·Molecular Cancer·Pan WangJing Liu
Sep 17, 2017·The Journal of Biological Chemistry·Risa Ebina-ShibuyaKazuhiko Igarashi
Jan 21, 2021·Signal Transduction and Targeted Therapy·Zi WangJing Liu
Sep 26, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Yvonne SundströmLouise Berg
Apr 22, 2021·Free Radical Biology & Medicine·Mei WangJulia Li Zhong
May 20, 2021·Nature Communications·Stefano GnanSara C B Buonomo
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