Epigenetic regulation of varicella-zoster virus open reading frames 62 and 63 in latently infected human trigeminal ganglia.

Journal of Virology
Lee GaryR J Cohrs

Abstract

Open reading frames (ORFs) 21, 29, 62, 63, and 66 of varicella-zoster virus (VZV) are transcribed during latency in human ganglia. ORF 63 is the most frequently expressed gene, and ORF 62 encodes a transcriptional activator. The mechanisms regulating the expression of these genes are not well understood, although analyses of other alphaherpesviruses indicate a role for chromatin in virus gene regulation during latent infection. Using chromatin immunoprecipitation (ChIP) assays to analyze the euchromatic state of ORFs 62 and 63 compared to the centromere from human chromosome 4 (heterochromatic) and the human glyceraldehyde-3-phosphate dehydrogenase promoter (euchromatic), we show that the promoters of ORFs 62 and 63 are associated with the histone protein H3K9(Ac) and thus maintained in a euchromatic state during latency. Conversely, the promoters of ORF 36 (thymidine kinase) and ORF 14 (glycoprotein C), genes expressed during lytic but not latent infection, were not enriched in the fraction of latently infected ganglia that bound to anti-H3K9(Ac) antibody. A ChIP assay using productively infected MeWo cells revealed that VZV ORFs 62, 63, 36, and 14 are all euchromatic. Together, these data indicate that the expression of the t...Continue Reading

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Citations

Jul 26, 2013·Current Neurology and Neuroscience Reports·Don GildenRavi Mahalingam
Jun 29, 2012·Journal of Virology·Werner J D OuwendijkGeorges M G M Verjans
Dec 24, 2010·Journal of Virology·Maria A NagelRandall J Cohrs
Jun 14, 2012·Herpesviridae·Paul R KinchingtonRobert L Hendricks
Dec 1, 2009·Future Neurology·Don GildenRandall J Cohrs
Sep 7, 2013·Viruses·Nicholas L BairdDon Gilden
Jul 1, 2007·Pakistan Journal of Biological Sciences : PJBS·Sara GharaviFarzaneh Sabahi
Aug 2, 2012·Journal of Neurovirology·Yevgeniy AzarkhRandall J Cohrs
Feb 24, 2011·Neuropathology and Applied Neurobiology·D GildenR J Cohrs
May 4, 2011·Reviews in Medical Virology·Michael NevelsChristina Paulus
Sep 27, 2014·The Journal of Pathology·Werner Jd Ouwendijk, Georges Mgm Verjans
Jan 22, 2010·The Journal of General Virology·Niklaus H MuellerRandall J Cohrs
Mar 22, 2015·The Journal of General Virology·Peter G E KennedyRandall J Cohrs
Jan 30, 2019·Viruses·Nicholas L BairdAbel Viejo-Borbolla
Feb 27, 2016·F1000Research·Don GildenNicholas Baird
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Jul 1, 2018·Viruses·Daniel P DepledgeWerner J D Ouwendijk
May 5, 2020·The Journal of Clinical Investigation·Jeffrey I Cohen
Sep 30, 2010·Journal of Neurovirology·Peter G E Kennedy, Randall J Cohrs
Jun 25, 2021·Frontiers in Microbiology·Ying WuAnchun Cheng

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