PMID: 9663659Jul 15, 1998Paper

Epigenetic reprogramming of the human H19 gene in mouse embryonic cells does not erase the primary parental imprint

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
K MitsuyaM Oshimura

Abstract

Genomic imprinting in mammals is thought to result from epigenetic modifications to chromosomes during gametogenesis, which leads to differential allelic expression during development. There is a requirement for an appropriate experimental system to enable the analysis of the mechanisms of genomic imprinting during embryogenesis. To develop a novel in vitro system for studying the molecular basis of genomic imprinting, we constructed mouse cell lines containing either a paternal or maternal human chromosome 11, by microcell-mediated chromosome transfer. Allele-specific expression and DNA methylation studies revealed that the imprinting status of the human H19 gene was maintained in mouse A9 mono-chromosomal hybrids. Each parental human chromosome was introduced independently into mouse near-diploid immortal fibroblasts (m5S) and two embryonal carcinoma (EC) cell lines (OTF9-63 and P19). The paternal allele of human H19 remained in a repressed state in m5S cells, but was de-repressed in both EC cells. The paternal H19 allele was demethylated extensively in OTF9-63 cells, whereas the only alteration in P19 hybrids was de novo methylation on both alleles in the 3' region. Following in vitro differentiation, the expressed paternal ...Continue Reading

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Citations

Mar 8, 2000·Environmental Health Perspectives·R L JirtleJ C Barrett
Jul 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Patrick OnyangoAndrew P Feinberg
Jun 9, 2001·Molecular Carcinogenesis·S MaegawaM Oshimura

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