Epigenetic signatures associated with different levels of differentiation potential in human stem cells.

PloS One
Pablo ArandaFelipe Prosper

Abstract

The therapeutic use of multipotent stem cells depends on their differentiation potential, which has been shown to be variable for different populations. These differences are likely to be the result of key changes in their epigenetic profiles. to address this issue, we have investigated the levels of epigenetic regulation in well characterized populations of pluripotent embryonic stem cells (ESC) and multipotent adult stem cells (ASC) at the trancriptome, methylome, histone modification and microRNA levels. Differences in gene expression profiles allowed classification of stem cells into three separate populations including ESC, multipotent adult progenitor cells (MAPC) and mesenchymal stromal cells (MSC). The analysis of the PcG repressive marks, histone modifications and gene promoter methylation of differentiation and pluripotency genes demonstrated that stem cell populations with a wider differentiation potential (ESC and MAPC) showed stronger representation of epigenetic repressive marks in differentiation genes and that this epigenetic signature was progressively lost with restriction of stem cell potential. Our analysis of microRNA established specific microRNA signatures suggesting specific microRNAs involved in regulat...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
May 22, 2002·The Journal of Clinical Investigation·Robert E SchwartzCatherine M Verfaillie
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Nov 3, 2004·Genes & Development·Giuseppina CarettiVittorio Sartorelli
Feb 17, 2005·Genes & Development·Chryssa KanellopoulouKlaus Rajewsky
Jun 9, 2005·Nature Biotechnology·Lisa M Hoffman, Melissa K Carpenter
Feb 2, 2006·Genome Research·Marina BibikovaJian-Bing Fan
Feb 16, 2006·Cell Cycle·Maria A LagarkovaSergei L Kiselev
Mar 30, 2006·Nature Cell Biology·Véronique AzuaraAmanda G Fisher
Apr 19, 2006·Genes & Development·Adrian P BrackenKristian Helin
Aug 11, 2006·Genome Research·Marina BibikovaJian-Bing Fan
Jan 18, 2007·Journal of Cellular Physiology·Antonio GiordanoIgnazio R Marino
Feb 20, 2007·Cancer Research·Amaia LujambioManel Esteller
Feb 27, 2007·Cell·M Azim SuraniPetra Hajkova
Mar 7, 2007·Molecular and Cellular Biology·Diego PasiniKristian Helin
Jun 6, 2007·Biochemical and Biophysical Research Communications·Seungeun YeoYong-Kook Kang
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Jul 3, 2007·Nature·Tarjei S MikkelsenBradley E Bernstein
Aug 29, 2007·Nature Biotechnology·Alexander MeissnerRudolf Jaenisch
Sep 6, 2007·The Journal of Cell Biology·Vincent van den BoomBart J L Eggen
Nov 3, 2007·Genes & Development·Hitoshi Niwa
Nov 23, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jose Roman-GomezXabier Agirre
Jan 4, 2008·The Journal of Clinical Investigation·Xabier L ArangurenAernout Luttun
Feb 26, 2008·Cell·Bradford M Stadler, Hannele Ruohola-Baker
Mar 29, 2008·Cell Stem Cell·Marina BibikovaJian-Bing Fan
Mar 29, 2008·Cell Stem Cell·Qintong Li, Richard I Gregory

❮ Previous
Next ❯

Citations

Jul 17, 2010·Regenerative Medicine·Kevin Huang, Guoping Fan
Sep 26, 2013·Tissue Engineering. Part B, Reviews·Wen-Jun HeXiao-Bing Fu
Jun 28, 2011·Stem Cell Reviews and Reports·Shizuka UchidaThomas Braun
Oct 21, 2010·Stem Cell Research & Therapy·Stephane Boeuf, Wiltrud Richter
Nov 4, 2010·Human Molecular Genetics·Arturo López CastelChristopher E Pearson
May 27, 2011·Genome Research·Agustin F FernandezManel Esteller
Oct 12, 2013·The AAPS Journal·Yuanyuan LiTrygve O Tollefsbol
Aug 7, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Iñigo IzalFelipe Prósper
Mar 1, 2014·Iranian Red Crescent Medical Journal·Najmaldin SakiAbdolreza Ardeshiry Lajimi
Apr 9, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Linda AttigClaudine Junien
Jan 15, 2013·Differentiation; Research in Biological Diversity·V B Fernández ValloneN A Chasseing
May 25, 2010·Biochimica Et Biophysica Acta·Marilena V IorioCarlo M Croce
Mar 5, 2016·Nucleic Acids Research·Shovamayee MaharanaG V Shivashankar
Dec 1, 2015·Frontiers in Bioengineering and Biotechnology·Vikram RaoShyni Varghese
Jan 5, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·D Adam YoungKaren L Christman
Feb 10, 2011·Stem Cells·Valerie D RoobrouckCatherine M Verfaillie
Dec 6, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jan M Friedman
Sep 9, 2010·Essays in Biochemistry·Shai Melcer, Eran Meshorer
Oct 8, 2014·Molecular and Cellular Endocrinology·Maximiliaan SchillebeeckxDavid B Wilson
Sep 18, 2012·Molecular Aspects of Medicine·Kyle J Hewitt, Jonathan A Garlick
Dec 17, 2014·Molecular and Cellular Endocrinology·Theresa RöhrigDavid B Wilson
Aug 1, 2013·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Mar 20, 2018·Journal of Neuroinflammation·Supinder S BediCharles S Cox
Aug 3, 2018·Stem Cells Translational Medicine·Mitchell J GeorgeCharles S Cox
Apr 20, 2018·Stroke; a Journal of Cerebral Circulation·Robert W Mays, Sean I Savitz
Dec 7, 2011·Leukemia·X AgirreF Prósper
Apr 9, 2020·Biomolecules·Elena Sánchez-LuisJavier De Las Rivas
Aug 26, 2016·Science Translational Medicine·Huan LiuJing Yang
Feb 1, 2019·Journal of Neuroinflammation·Zhe WangXiao-Jun Tan
Apr 8, 2020·Clinical Epigenetics·Carolina SerenaSonia Fernández-Veledo

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Methods Mentioned

BETA
methylation profiling
PCA
PCR
Immunoprecipitation
ChIP

Software Mentioned

Significant Analysis of Microarrays ( SAM )
SAM
LIMMA
TIGR MeV

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