Epigenome-wide analysis in newborn blood spots from monozygotic twins discordant for cerebral palsy reveals consistent regional differences in DNA methylation.

Clinical Epigenetics
Namitha MohandasJeffrey M Craig

Abstract

Cerebral palsy (CP) is a clinical description for a group of motor disorders that are heterogeneous with respect to causes, symptoms and severity. A diagnosis of CP cannot usually be made at birth and in some cases may be delayed until 2-3 years of age. This limits opportunities for early intervention that could otherwise improve long-term outcomes. CP has been recorded in monozygotic twins discordant for the disorder, indicating a potential role of non-genetic factors such as intrauterine infection, hypoxia-ischaemia, haemorrhage and thrombosis. The aim of this exploratory study was to utilise the discordant monozygotic twin model to understand and measure epigenetic changes associated with the development of CP. We performed a genome-wide analysis of DNA methylation using the Illumina Infinium Human Methylation 450 BeadChip array with DNA from newborn blood spots of 15 monozygotic twin pairs who later became discordant for CP. Quality control and data preprocessing were undertaken using the minfi R package. Differential methylation analysis was performed using the remove unwanted variation (RUVm) method, taking twin pairing into account in order to identify CP-specific differentially methylated probes (DMPs), and bumphunter w...Continue Reading

References

Sep 9, 1989·Lancet·D J BarkerS J Simmonds
Jul 1, 1994·Pediatric Pathology·S M Jacques, F Qureshi
Apr 10, 1993·Lancet·D J BarkerJ S Robinson
Jun 1, 1993·Developmental Medicine and Child Neurology·A Leviton
Apr 1, 1996·Annals of Neurology·R OttmanM Susser
Oct 1, 1995·Seminars in Perinatology·K Benirschke
Apr 12, 2001·Arquivos de neuro-psiquiatria·I BruckC G Pacheco
Jan 5, 2002·Journal of Physiology, Paris·Kerstin KrieglsteinKlaus Unsicker
Mar 14, 2002·Brain & Development·Adriana MarbiniF Gemignani
Oct 5, 2002·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Hazim Kadhim, Guillaume Sébire
Nov 5, 2002·Nature Reviews. Genetics·Dorret BoomsmaLeena Peltonen
Nov 12, 2003·The Journal of Experimental Medicine·Eun Mi HurYungdae Yun
Feb 19, 2005·Pediatric Research·Karin B NelsonEdward Lammer
Jun 4, 2005·Obstetrics and Gynecology·Florence LivinecUNKNOWN Epipage Group
Mar 16, 2006·Current Opinion in Neurology·John M Keogh, Nadia Badawi
Aug 1, 2006·Neuroscience Letters·Toshimitsu SuzukiKazuhiro Yamakawa
Mar 21, 2007·Developmental Medicine and Child Neurology. Supplement·Christopher Morris
Nov 14, 2007·American Journal of Human Genetics·Christine Ladd-AcostaAndrew P Feinberg
Nov 4, 2008·Pediatrics·Catherine S GibsonKarin B Nelson
Feb 17, 2009·Pediatric Neurology·Sylvie GirardGuillaume Sébire
Feb 25, 2009·Human Genetics·M E O'CallaghanUNKNOWN South Australian Cerebral Palsy Research Group
May 21, 2009·Human Reproduction Update·P O D Pharoah, Y Dundar
Jul 1, 2009·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Susanne Holst RavnPeter Uldall
Jul 17, 2009·Journal of Child Neurology·Shadi Malaeb, Olaf Dammann
Mar 2, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Lavinia GordonJeffrey M Craig
Jul 13, 2011·Nature Reviews. Genetics·Vardhman K RakyanStephan Beck
Mar 17, 2012·International Journal of Epidemiology·Andrew E JaffeRafael A Irizarry
May 10, 2012·BMC Bioinformatics·Eugene Andres HousemanKarl T Kelsey
May 23, 2012·Lancet Neurology·Bobbi Fleiss, Pierre Gressens
Aug 1, 2012·Nature Reviews. Genetics·Jenny van DongenDorret I Boomsma
Sep 4, 2012·International Journal of Molecular Sciences·Arpád DobolyiGábor Lovas
Oct 5, 2012·Genome Biology·Steve HorvathRoel A Ophoff
Oct 10, 2012·Pediatrics·Iona NovakRichard Barclay
Nov 8, 2012·Genome Medicine·Mark N CruickshankJeffrey M Craig
Nov 29, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Lucas R SmithRichard L Lieber

❮ Previous
Next ❯

Citations

Jan 28, 2020·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Jeffrey M CraigDedra Buchwald
Dec 11, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Yu JiangWilfried Karmaus
Aug 29, 2019·International Journal of Molecular Sciences·Marina La RovereLiborio Stuppia
May 1, 2019·International Journal of Molecular Sciences·Ray O Bahado-SinghUppala Radhakrishna
Dec 18, 2020·Journal of Neurodevelopmental Disorders·Ana G Cristancho, Eric D Marsh
Feb 9, 2021·Frontiers in Neurology·Sara A LewisMichael C Kruer
Jun 10, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Philip J LupoMichael E Scheurer
Jul 21, 2021·World Journal of Pediatrics : WJP·Fang-Fang WangDe-Zhi Mu
Aug 28, 2021·Journal of Personalized Medicine·Stephanie LeeRobert Akins
Aug 27, 2021·Frontiers in Pediatrics·John AllenEleanor J Molloy
Sep 25, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lydia A SibleyAndrea A Domenighetti
Nov 28, 2021·Nature Reviews. Genetics·Sean WhalenKatherine S Pollard

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
chip
biopsies

Software Mentioned

MassArray EpiTYPER
minfi
bumphunter
RUVm
missMethyl
Sequenom EpiDesigner
missMethyl R package
Bioconductor
BiocLite MassArray package
missMethyl package

Related Concepts

Related Feeds

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.