Epinastine hydrochloride antagonism against interleukin-4-mediated T cell cytokine imbalance in vitro

International Archives of Allergy and Immunology
Ken-Ichi KanaiHarumi Suzaki

Abstract

Interleukin (IL)-4 is well accepted to be a cytokine that plays many roles in the regulation of immune responses. Although the primary pharmacological target of antihistamines has been regarded as the histamine H1 receptor, there is little information about the influence of antihistamines on IL-4-mediated immune responses. The present study was undertaken to examine whether H1 receptor antagonists could modulate IL-4-mediated immune responses in vitro. CD4+ T cells from normal human peripheral blood (1 x 10(6) cells/ml) were incubated with various concentrations of epinastine hydrochloride (EP) or chlorpheniramine (CH) for 30 min and then stimulated with 10.0 ng/ml IL-4. After 24 h, culture supernatants were collected and assayed for IL-5, IL-6, IL-13 and interferon-gamma by ELISA. The influence of EP on transcription factor activation and mRNA expression for cytokines was also examined. Addition of EP into cell cultures at more than 20.0 ng/ml significantly suppressed the production of IL-5, IL-6 and IL-13, which were increased by IL-4 stimulation. EP at more than 20.0 ng/ml also suppresses nuclear factor-kappaB activation, signal transducers and activators of transcription 6 phosphorylation and mRNA expression, which were upr...Continue Reading

Citations

Jul 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Misako Sakai-Kashiwabara, Kazuhito Asano
Feb 4, 2014·BioMed Research International·Toshimitsu KomatsuzakiHarumi Suzaki
Jul 28, 2009·Expert Opinion on Drug Metabolism & Toxicology·Shilpi PradhanFrancis Mah
May 25, 2010·Neuropharmacology·Farhad Jadidi-Niaragh, Abbas Mirshafiey
Sep 20, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Grazyna GalatowiczVirginia L Calder
Jan 21, 2014·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Pakit VichyanondAndrea Leonardi
Oct 25, 2006·International Ophthalmology Clinics·William B TrattlerParag Majmudar
Aug 27, 2013·Current Opinion in Allergy and Clinical Immunology·Punchama Pacharn, Pakit Vichyanond
Aug 6, 2020·Medicines·Yoshihito TanakaHitome Kobayashi

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