Abstract
All plakin family proteins are known to be autoantigens in paraneoplastic pemphigus (PNP). In this study, we first examined whether PNP sera also react with epiplakin, another plakin protein, by various immunological methods using 48 Japanese PNP sera. Immunofluorescence confirmed that cultured keratinocytes expressed epiplakin. Epiplakin was detected by 72.9% of PNP sera by immunoprecipitation-immunoblotting with KU-8 cell extract, but not by immunoblotting of either normal human epidermal extract or KU-8 cell extract. Epiplakin was essentially not detected by 95 disease and normal control sera. Statistical analyses of various clinical and immunological findings revealed a significant correlation of the presence of anti-epiplakin antibodies with both bronchiolitis obliterans and mortality. No epiplakin-negative PNP case developed bronchiolitis obliterans. However, although 29.4% of European patients with PNP had bronchiolitis obliterans, significant correlation with anti-epiplakin autoantibodies was not observed. In further studies for lung, immunofluorescence showed the presence of epiplakin in normal human lung, particularly respiratory bronchiole, immunoprecipitation-immunoblotting showed that PNP sera reacted with epiplaki...Continue Reading
Citations
Jul 18, 2019·American Journal of Clinical Dermatology·Hanan RashidBarbara Horváth
Sep 12, 2019·Paediatric Drugs·Brittney Schultz, Kristen Hook
Nov 20, 2018·Frontiers in Medicine·Mareike WitteEnno Schmidt
Jun 20, 2019·Frontiers in Immunology·Jong Hoon Kim, Soo-Chan Kim
May 12, 2017·Nature Reviews. Disease Primers·Michael KasperkiewiczMasayuki Amagai
Nov 24, 2016·American Journal of Clinical Dermatology·Saritha KartanLawrence S Chan
Sep 10, 2017·Experimental Dermatology·Stephanie GoletzEnno Schmidt
Feb 19, 2017·The British Journal of Dermatology·K OkahashiA Kawada
Feb 23, 2018·Nature Reviews. Disease Primers·Kyle T Amber
Jul 12, 2019·Frontiers in Immunology·Dario DidonaMichael Hertl
Jul 21, 2020·Case Reports in Pathology·Kentaro OdaniYutaka Tsutsumi
Dec 27, 2018·The British Journal of Dermatology·S SunX Zeng
Oct 24, 2019·Anais Brasileiros De Dermatologia·Celina Wakisaka MarutaClaudia Giuli Santi
Oct 30, 2016·The British Journal of Dermatology·T HashimotoN Ishii
Feb 27, 2018·Immunologic Research·Khalaf Kridin
Dec 1, 2017·International Journal of Molecular Sciences·Giovanni PaolinoStefano Calvieri
Jan 1, 2019·Anais Brasileiros De Dermatologia·Celina Wakisaka MarutaClaudia Giuli Santi
Sep 10, 2019·Lancet·Enno SchmidtPascal Joly
Jan 23, 2016·The British Journal of Dermatology·T HashimotoN Ishii
Mar 24, 2021·Annals of Hematology·Tomohiro KishiMasaru Shibano
May 21, 2021·Pediatric Transplantation·Sander A R FidderHubert P J van der Doef
Jun 11, 2021·Bone Marrow Transplantation·Nozomu KawashimaYoshiyuki Takahashi
Jul 20, 2021·Journal of Dermatological Science·Daisuke UeoSakuhei Fujiwara
Oct 12, 2021·Experimental and Therapeutic Medicine·Victor-Vlad CostanMihaela Paula Toader