Epiregulin promotes the migration and chemotaxis ability of adipose-derived mesenchymal stem cells via mitogen-activated protein kinase signaling pathways

Journal of Cellular Biochemistry
Yangyang CaoZhipeng Fan

Abstract

To investigate the function of epiregulin (EREG) in the migration and chemotaxis ability of mesenchymal stem cells. Adipose-derived stem cells (ADSCs) were used in this investigation. Lentiviral EREG short hairpin RNA was applied to silence EREG expression in ADSCs. Human recombinant EREG protein (rhEREG) was used to perform a gain-of-function study. Scratch-simulated wound migration and transwell chemotaxis assays were used to examine the migration and chemotaxis capacity of ADSCs in vitro. Using a Western blot assay, the expressions of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun N-terminal kinase (JNK), extracellular signal-regulated protein kinases 1 and 2 (Erk1/2), and protein kinase B were detected. Depletion of EREG caused by specific short hairpin RNA restrained the migration and chemotaxis ability of ADSCs and inhibited the expressions of phosphorylated p38 MAPK, JNK, and Erk1/2. rhEREG improved ADSCs migration and chemotaxis capacity, which was repressed by knockdown of EREG and rescued the expressions of phosphorylated p38 MAPK, JNK, and Erk1/2 impaired by silencing EREG. Furthermore, rhEREG-improved migration and chemotaxis ability in EREG-depleted-ADSCs was restricted by a specific inhibitor, SB203580, fo...Continue Reading

References

Aug 5, 2003·Journal of Cellular Biochemistry·Bradley K DraperLillian B Nanney
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Philippe P Roux, John Blenis
Jul 11, 2006·Nature Reviews. Molecular Cell Biology·Ami Citri, Yosef Yarden
Mar 14, 2007·Stem Cells and Development·Yoshie OzakiYukio Kato
Jun 14, 2008·American Journal of Physiology. Cell Physiology·Chhanda Bose, Kodetthoor B Udupa
Nov 27, 2008·Archives of Virology·Xiaoran DingShengqi Wang
Jul 7, 2009·Nature Cell Biology·Zhipeng FanCun-Yu Wang
Sep 4, 2010·Differentiation; Research in Biological Diversity·Gaëtan J-R DelcroixClaudia N Montero-Menei
Mar 20, 2012·Investigative Ophthalmology & Visual Science·Yuan ZhangYuichi Ohashi
Nov 7, 2013·Stem Cells International·Abhishek Sohni, Catherine M Verfaillie
Dec 19, 2013·Genes & Cancer·Vasudha Sehgal, Prahlad T Ram
Feb 12, 2014·Seminars in Cell & Developmental Biology·Fenghua Zeng, Raymond C Harris
Mar 19, 2014·Seminars in Cell & Developmental Biology·David J Riese, Richard L Cullum
Dec 30, 2014·Expert Opinion on Biological Therapy·Karlien Kallmeyer, Michael S Pepper
Feb 10, 2016·Biotechnology Letters·Dong-Hun WooJoong Hyun Shim
Mar 30, 2016·World Journal of Stem Cells·Ann De Becker, Ivan Van Riet
Oct 11, 2016·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Laura FreseSimon P Hoerstrup
Mar 10, 2017·Biochemical and Biophysical Research Communications·Mariko IijimaYoshikazu Shibasaki

❮ Previous
Next ❯

Citations

Nov 21, 2019·Disease Models & Mechanisms·Christine LehnerAndreas Traweger
Dec 28, 2019·International Journal of Molecular Sciences·Nahyun ChoiJong-Hyuk Sung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.