Epiregulin stimulates proliferation of rabbit gastric cells in primary culture through autophosphorylation of the epidermal growth factor receptor

European Journal of Pharmacology
E SasakiK Kobayashi

Abstract

Epiregulin, a growth factor of the epidermal growth factor (EGF) family, was recently purified from conditioned medium of a mouse fibroblast-derived tumor cell line. It was reported that epiregulin exhibited bifunctional properties in the regulation of cell growth. However, the effect of epiregulin on gastric cell proliferation is not known. The aims of this study were to determine whether: (1) epiregulin affects proliferation of rabbit cultured gastric cells, (2) epiregulin-induced stimulation of cell proliferation is mediated by the tyrosine kinase pathway, and (3) epiregulin stimulates autophosphorylation of EGF-receptors. Epiregulin stimulated cell proliferation to a significant extent. This effect was completely blocked by treatment with genistein. Epiregulin stimulated tyrosine phosphorylation of a 170 kDa protein, which represents the EGF receptor, in a dose-dependent fashion. These findings suggest that epiregulin has mitogenic effects on rabbit gastric cultured cells, possibly mediated via the tyrosine kinase pathway through autophosphorylation of EGF receptors.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1990·Gastroenterologia Japonica·S OtaT Sugimoto
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M H KrausS A Aaronson
Jan 1, 1987·Annual Review of Biochemistry·G Carpenter
Mar 31, 1995·The Journal of Biological Chemistry·H ToyodaK Hanada
Jul 1, 1994·Digestive Diseases and Sciences·K YoshiuraK Okano
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G D PlowmanM Shoyab
Mar 15, 1993·Biochemical and Biophysical Research Communications·M TakahashiM Omata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.