Epithelial basement membrane injury and regeneration modulates corneal fibrosis after pseudomonas corneal ulcers in rabbits

Experimental Eye Research
Gustavo K MarinoSteven E Wilson

Abstract

The purpose of this study was to investigate whether myofibroblast-related fibrosis (scarring) after microbial keratitis was modulated by the epithelial basement membrane (EBM) injury and regeneration. Rabbits were infected with Pseudomonas aeruginosa after epithelial scrape injury and the resultant severe keratitis was treated with topical tobramycin. Corneas were analyzed from one to four months after keratitis with slit lamp photos, immunohistochemistry for alpha-smooth muscle actin (α-SMA) and monocyte lineage marker CD11b, and transmission electron microscopy. At one month after keratitis, corneas had no detectible EBM lamina lucida or lamina densa, and the central stroma was packed with myofibroblasts that in some eyes extended to the posterior corneal surface with damage to Descemet's membrane and the endothelium. At one month, a nest of stromal cells in the midst of the SMA + myofibroblasts in the stroma that were CD11b+ may be fibrocyte precursors to myofibroblasts. At two to four months after keratitis, the EBM fully-regenerated and myofibroblasts disappeared from the anterior 60-90% of the stroma of all corneas, except for one four-month post-keratitis cornea where anterior myofibroblasts were still present in one lo...Continue Reading

Citations

Nov 28, 2018·Investigative Ophthalmology & Visual Science·Paramananda SaikiaSteven E Wilson
Aug 30, 2018·Scientific Reports·Kye-Im JeonKrystel R Huxlin
Jan 10, 2018·Journal of Refractive Surgery·Steven E WilsonMarcony R Santhiago
Oct 5, 2018·Cell and Tissue Research·Paramananda SaikiaSteven E Wilson
Aug 12, 2018·Investigative Ophthalmology & Visual Science·Carla S MedeirosSteven E Wilson
Mar 19, 2019·Investigative Ophthalmology & Visual Science·Carla S MedeirosSteven E Wilson
Aug 24, 2018·Scientific Reports·Pouriska B KivananyW Matthew Petroll
Oct 18, 2020·Experimental Eye Research·Steven E WilsonRodrigo Carlos de Oliveira
Feb 27, 2018·Experimental Eye Research·Luciana LassanceSteven E Wilson
Jun 20, 2020·Experimental Eye Research·Steven E Wilson
Oct 4, 2020·Experimental Eye Research·Steven E Wilson
Mar 18, 2020·Experimental Eye Research·Steven E WilsonGustavo K Marino
Sep 24, 2018·Experimental Eye Research·Patricia Gallego-MuñozM Carmen Martínez-García
Apr 25, 2021·Experimental Eye Research·Steven E Wilson
Jun 8, 2021·Experimental Eye Research·Lawson Ung, James Chodosh
Jul 8, 2021·Investigative Ophthalmology & Visual Science·Ye LiPengXia Wan
Sep 7, 2021·Journal of Cellular and Molecular Medicine·Nina SeidelmannMatthias Fuest

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