Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas

Experimental Eye Research
Andre A M TorricelliS E Wilson

Abstract

The epithelial basement membrane (BM) is a specialized extracellular matrix that has been shown to have a critical role in corneal development, wound healing, and disease. Although the epithelial BM contributes to corneal homeostasis, relatively little is know about non-epithelial production of its components that may be important in defective regeneration of the epithelial basement membrane associated with opacity after photorefractive keratectomy. The purpose of the current study was to investigate stromal production of corneal epithelial BM proteins in wounded human corneas using immunohistochemistry. A total of five unwounded control eyes and five 30-min epithelial-wounded corneas were obtained from fresh corneoscleral buttons removed from human eyes enucleated due to choroidal melanoma with normal anterior segments. In the wounded corneas, an eight mm patch of central corneal epithelium and epithelial BM was removed with a Beaver blade when the patient was under general anesthesia. Immunohistochemical analyses were performed to detect perlecan and nidogen-2 proteins-important components of the epithelial BM lamina lucida and lamina densa zones. Perlecan and nidogen-2 proteins were detected in the BM itself and at low level...Continue Reading

References

Dec 15, 1983·European Journal of Biochemistry·R TimplG Wick
Aug 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M P MarinkovichR E Burgeson
Apr 7, 1999·Progress in Retinal and Eye Research·S E WilsonR R Mohan
Feb 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Erhard Hohenester, Jürgen Engel
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Brian M StramerM Elizabeth Fini
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Peter D YurchencoBruce L Patton
Jul 29, 2004·Journal of Cellular Physiology·Jason Vittitow, Teresa Borrás
Jul 19, 2005·Molecular and Cellular Biology·Bernhard L BaderRoswitha Nischt
Nov 5, 2005·The Journal of Biological Chemistry·Ifat SherDina Ron
Nov 24, 2005·Experimental Eye Research·Marcelo V NettoSteven E Wilson
Mar 22, 2006·Experimental Eye Research·Simone Smith, John R Hassell
Jan 26, 2008·Microscopy Research and Technique·Matthew S P HoNeil Smyth
Jun 16, 2009·Experimental Eye Research·Renato AmbrósioSteven E Wilson
Apr 30, 2010·Cellular and Molecular Life Sciences : CMLS·Jenny Kruegel, Nicolai Miosge
Jan 24, 2012·Investigative Ophthalmology & Visual Science·Takenori InomataEri Arikawa-Hirasawa
May 23, 2013·Investigative Ophthalmology & Visual Science·Andre A M TorricelliSteven E Wilson
Oct 1, 2013·Investigative Ophthalmology & Visual Science·André A M TorricelliSteven E Wilson
Oct 5, 2014·Experimental Eye Research·Andre A M Torricelli, Steven E Wilson

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Citations

Nov 28, 2018·Investigative Ophthalmology & Visual Science·Paramananda SaikiaSteven E Wilson
Jan 10, 2018·Journal of Refractive Surgery·Steven E WilsonMarcony R Santhiago
Oct 5, 2018·Cell and Tissue Research·Paramananda SaikiaSteven E Wilson
Aug 12, 2018·Investigative Ophthalmology & Visual Science·Carla S MedeirosSteven E Wilson
Nov 20, 2019·Scientific Reports·Nimisha R KumarArkasubhra Ghosh
Sep 10, 2020·Frontiers in Cell and Developmental Biology·Sudan PuriVivien J Coulson-Thomas
Jun 20, 2020·Experimental Eye Research·Steven E Wilson
Apr 28, 2020·Experimental Eye Research·Steven E Wilson
Mar 18, 2020·Experimental Eye Research·Steven E WilsonGustavo K Marino
Jul 4, 2020·Experimental Eye Research·Tina B McKayDimitrios Karamichos
Sep 24, 2018·Experimental Eye Research·Patricia Gallego-MuñozM Carmen Martínez-García
Aug 3, 2021·Analytical Cellular Pathology (Amsterdam)·Tina B McKayJoseph B Ciolino

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