Epithelial cells in culture: injured or differentiated cells?

Cell Biology International
Patrick C Baer, Jurgen Bereiter-Hahn

Abstract

Isolation of epithelial cells for cell culture is based on destruction of epithelial integrity. The consequences are manifold: cell polarity and specific cell functions are lost; cells acquire non-epithelial characteristics and start to proliferate. This situation may also occur in situ when parts of the epithelium are lost, either by apoptosis or necrosis by organ or tissue injury. During recovery from this injury, surviving epithelial cells proliferate and may restore epithelial integrity and finally re-differentiate into functional epithelial cells. In vitro, this re-differentiation is mostly not complete due to sub-optimal culture conditions. Therefore cultured epithelial cells resemble wounded or injured epithelia rather than healthy and well differentiated epithelia. The value of an in vitro cell model is the extent to which it helps to understand the function of the cells in situ. A variety of parameters influence the state of differentiation of cultured cells in vitro. Although each of these parameters had been studied, the picture how they co-ordinately influence the state of differentiation of epithelial cells in vitro is incomplete. Therefore we discuss the influence of the isolation method and cell culture on epithe...Continue Reading

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Citations

Aug 26, 2014·Experimental Eye Research·Bruce A Pfeffer, Nancy J Philp
Sep 27, 2014·Acta Medica (Hradec Králové)·Jürgen Bereiter-HahnNadja Zöller
Apr 1, 2017·American Journal of Respiratory Cell and Molecular Biology·Tanyalak Parimon, Peter Chen
Jun 13, 2018·International Journal of Molecular Sciences·Patrick C BaerHelmut Geiger
Aug 7, 2019·The International Journal of Artificial Organs·Natalia Sánchez-RomeroIgnacio Giménez

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