Epithelial cyclooxygenase-2 expression: a model for pathogenesis of colon cancer

The Journal of Surgical Research
S ArbabiR V Maier

Abstract

Recent studies indicate a close relationship between cyclooxygense-2 (COX-2) expression and the pathogenesis of colorectal cancer, yet little information exists regarding the stimuli and pathways involved in COX-2 expression by the colonic epithelium. We studied the induction of COX-2 in response to such environmental stress as hyperosmolarity and lipopolysaccharide (LPS) in a human colon cell line. We further investigated the transduction cascades mediating COX-2 expression, focusing upon the mitogen-activated protein kinase pathways p38 and extracellular signal-regulated kinase (ERK). Human colon cancer cells (Caco-2) were stimulated with increasing concentrations of sodium chloride (NaCl) or LPS. Total protein was extracted at different time points and subjected to Western blot analysis with antibodies to human COX-2, COX-1, or phospho-specific antibodies to ERK and p38. LPS failed to induce COX-2 or COX-1 expression. Hyperosmolarity induced COX-2 expression by 2 h, with peak levels occurring at 6-8 h. NaCl at 40 and 100 mM induced a 2-fold and more than 50-fold increase in COX-2 expression, respectively; COX-1 expression was not affected. Hyperosmolarity induced both p38 and ERK activation within 30 min; however, only p38 i...Continue Reading

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Citations

Aug 31, 2007·Biochemical and Biophysical Research Communications·Giulia CoronaJeremy P E Spencer
Jul 10, 2007·Journal of Pediatric Surgery·Brian LugoAnatoly Grishin
Dec 5, 2006·European Journal of Pharmacology·Maria Eline MatheusPatricia Dias Fernandes
Aug 24, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J T NurmiN E Rautonen
Feb 7, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Donnie E ShifflettAnthony T Blikslager
Mar 30, 2007·The British Journal of Nutrition·Francesca FavaNina Rautonen
Feb 4, 2011·American Journal of Physiology. Cell Physiology·Min ChenKathleen L O'Connor
Aug 7, 2003·American Journal of Clinical Oncology·Asad UmarErnest T Hawk
Sep 24, 2005·The Journal of Nutrition·Pernilla C KarlssonJoseph J Rafter
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anatoly V GrishinHenri R Ford
Jan 14, 2017·The American Journal of Pathology·François BlachierDaniel Tomé

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